Anti-Syntaxin Antibody (SP8)

Name: Anti-Syntaxin Antibody
Brand: PhosphoSolutions
SKU: 1990-STX
Price: 456.0 USD
Availability: InStock

Our Anti-Syntaxin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, rat, pig, and hamster Syntaxin and is Protein G purified. It is great for use in WB, IHC.

Western blot of rat cortical lysate showing specific immunolabeling of the ~ 36 kDa syntaxin 1A protein.

Click on image to zoom

Species Reactivity

Hamster, Human, Pig, Rat

Applications

ELISA, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1990-STX

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Syntaxin

Scientific Background

Syntaxins are a family of proteins involved in the docking of synaptic vesicles with the plasma membrane. Syntaxin, along with synaptobrevin and SNAP-25, forms the SNARE complex which mediates synaptic vesicle fusion and exocytosis. Binding of Munc18-1 to the N-terminus of syntaxin 1 has been demonstrated to be essential for exocytic membrane fusion (Khvotchev et al., 2007). Decreased levels of phosphorylated syntaxin 1 in the prefrontal cortex have recently been correlated with schizophrenia (Castillo et al., 2010).

Format

Protein G Purified

Clonality

Monoclonal

Clone

SP8

Isotype

IgG1

Applications

ELISA, IHC, WB

Host Species

Mouse

Gene Name

STX1A

Molecular Weight

36 kDa

Immunogen

Synaptic immunoprecipitate (crude) from human brain.

Immunogen Species

Human

Species Reactivity

Hamster, Human, Pig, Rat

Antibody Registry ID

AB_2492254

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~36 kDa syntaxin protein. The antibody has also been used for immunohistochemistry on formalin fixed, vibratome sections. *Does not work on paraffin sections.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

HPC 1; Neuron specific antigen HPC1; brain; Neuron-specific antigen HPC-1; OTTHUMP00000174615; OTTHUMP00000174616; OTTHUMP00000174617; OTTHUMP00000174618; P35-1; STX1; STX1A; STX1A_HUMAN; SYN1A; Syntaxin 1A (brain; Syntaxin 1A brain; Syntaxin-1A; Syntaxin 1A

UniProt ID

Q16623
UniProt Summary: Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed:26635000). Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm (PubMed:23091057). Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) (By similarity). {ECO:0000250|UniProtKB:O35526, ECO:0000269|PubMed:23091057, ECO:0000269|PubMed:26635000}.

NCBI Gene ID

6804

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Blue Ice

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Anti-Syntaxin Antibody (SP8)

SKU: 1990-STX

Product Details

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