Anti-SARM1 Antibody

Name: Anti-SARM1 Antibody
Brand: PhosphoSolutions
SKU: 1901-SARM1
Price: 456.0 USD
Availability: InStock

Our Anti-SARM1 Antibody primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat SARM1 and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Immunofluorescence of transfected COS-7 cells specifically labeling the positive SARM1 cells using our anti-SARM1 antibody (1:50, red) and anti-FLAG antibody (green). The yellow color results from the overlap of cells labeled with both antibodies. DNA is labeled with DAPI (blue).

Western blot of HeLa cell lysate showing specific immunolabeling of the ~79 kDa SARM1 protein.

Immunofluorescence of transfected COS-7 cells specifically labeling the positive SARM1 cells using our anti-SARM1 antibody (1:50, red) and anti-FLAG antibody (green). The yellow color results from the overlap of cells labeled with both antibodies. DNA is labeled with DAPI (blue).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: 1901-SARM1

Size: 100 uL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

SARM1

Scientific Background

NAD(+) hydrolase sterile alpha and TIR motif-containing protein 1 (SARM1), is the fifth member of the Toll/Il-1 Receptor (TIR) domain protein family. The highly conserved 724 amino acid protein is predominantly expressed in the brain, kidney and liver (Mink, M., et al 2001). Furthermore, SARM1 is enriched in the cytoplasm, mitchondria, and the axons, dendrites, and synapses of neurons (Panneerselvam, P., et al, 2012). SARM1 plays a key role in axonal degeneration by catalyzing the cleavage of NAD+ into ADPR, cADPR, and nicotinamide in response to injury triggering Wallerian degeneration (Gerdts, J. et al 2015). SARM1 has also been shown to activate neuronal cell death in response to stress (Peng, J. et al. 2010).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

SARM1

Molecular Weight

79 kDa

Immunogen

Full length recombinant human SARM1 protein.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2922825

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the recombinant protein antigen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
ICC: 1:50

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~79 kDa SARM1 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

FLJ36296; KIAA0524; MyD88-5; SAM and ARM-containing protein; SAM domain-containing protein 2; SAMD2; SARM 1; SARM1; SARM1_HUMAN; Sterile alpha and Armadillo repeat protein; sterile alpha and HEAT/Armadillo motif protein; ortholog of Drosophila; sterile alpha and HEAT/Armadillo motifs-containing protein; sterile alpha and TIR motif containing 1; Sterile alpha and TIR motif-containing protein 1; Sterile alpha and TIR motifs-containing protein 1; Sterile alpha motif domain-containing protein 2; Tir-1 homolog

UniProt ID

Q6SZW1
UniProt Summary: NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism (PubMed:25908823, PubMed:27671644, PubMed:28334607). Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site (PubMed:15123841, PubMed:16964262, PubMed:20306472, PubMed:25908823). Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction (PubMed:25908823, PubMed:28334607, PubMed:30333228, PubMed:31128467, PubMed:31439792, PubMed:31439793, PubMed:32049506, PubMed:32828421, PubMed:33053563). Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules (PubMed:29395922). Can activate neuronal cell death in response to stress (PubMed:20306472). Regulates dendritic arborization through the MAPK4-JNK pathway (By similarity). Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PubMed:16964262). {ECO:0000250|UniProtKB:Q6PDS3, ECO:0000269|PubMed:15123841, ECO:0000269|PubMed:16964262, ECO:0000269|PubMed:20306472, ECO:0000269|PubMed:25908823, ECO:0000269|PubMed:27671644, ECO:0000269|PubMed:28334607, ECO:0000269|PubMed:29395922, ECO:0000269|PubMed:30333228, ECO:0000269|PubMed:31128467, ECO:0000269|PubMed:31439792, ECO:0000269|PubMed:31439793, ECO:0000269|PubMed:32049506, ECO:0000269|PubMed:32828421, ECO:0000269|PubMed:33053563}.

NCBI Gene ID

23098

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Anti-SARM1 Antibody

SKU: 1901-SARM1

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