Anti-Rabphilin 3A (Ser234) Antibody

Name: Anti-Rabphilin 3A (Ser234) Antibody
Brand: PhosphoSolutions
SKU: p1553-234
Price: 456.0 USD
Availability: InStock

Our Anti-Rabphilin 3A (Ser234) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Rabphilin 3A (Ser234) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Western blot of rat brain lysate showing specific immunolabeling of the ~82 kDa rabphilin 3A phosphorylated at Ser234.

Immunostaining of 14 DIV rat cortical neurons labeling rabphilin when phosphorylated at Ser234 (Cat. p1553-234, red, 1:400) and PSD95 (green). The rat cortical neurons were fixed with methanol at -20C for 10 minutes. Photo courtesy of Gang Liu.

Western blot of rat brain lysate showing specific immunolabeling of the ~82 kDa rabphilin 3A phosphorylated at Ser234.

Click on image to zoom

Species Reactivity

Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

Go to Full Product Details

Data Sheet

Trial Size

Pooled Serum

SKU: p1553-234

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Rabphilin 3A (Ser234)

Scientific Background

Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994; Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser-234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995; Foletti and Scheller, 2001).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

RPH3A

Molecular Weight

82 kDa

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of rat rabphilin 3A, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Rat

Expected Reactivity

Mouse

Antibody Registry ID

AB_2492222

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:400

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~82 kDa rabphilin protein phosphorylated at Ser234. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser234

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Exophilin 1; Exophilin-1; Exophilin1; KIAA0985; Rabphilin 3A homolog; Rabphilin-3A; Rabphilin3A; RP3A; RP3A_HUMAN; Rph 3a; Rph3a

UniProt ID

P47709
UniProt Summary: Plays an essential role in docking and fusion steps of regulated exocytosis (By similarity). At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release (By similarity). In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Also plays a role in the exocytosis of arginine vasopressin hormone (By similarity). {ECO:0000250|UniProtKB:P47709}.

NCBI Gene ID

171039

Shipping

Blue Ice

Western Blot Protocol: Download

Recover password

Create my account

Anti-Rabphilin 3A (Ser234) Antibody

SKU: p1553-234

Product Details

Bulk Order Anti-Rabphilin 3A (Ser234) Antibody

Related Products

Recently Viewed