Anti-PSD-95 Antibody

Name: Anti-PSD-95 Antibody
Brand: PhosphoSolutions
SKU: 1080-PSD95
Price: 456.0 USD
Availability: InStock

Our Anti-PSD-95 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat PSD-95 and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~95 kDa PSD-95 protein.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: 1080-PSD95

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

PSD-95

Scientific Background

PSD-95 is a very prominent component of the postsynaptic densities of synapses. It contains three PDZ domains which play key roles in its interactions with other proteins in the synapse. It has been proposed that these PDZ domains organize glutamate receptors and their associated signaling proteins and determine the size and strength of synapses (Kim and Sheng, 2004). Recent work suggests that interaction of the NMDAR with PSD-95 via these PDZ domains can be regulated by phosphorylation (Chung et al., 2004).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

DLG4

Molecular Weight

95 kDa

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat PSD-95, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Human, Non-Human Primate, Zebrafish

Antibody Registry ID

AB_2492218

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column with the N-terminal peptide used as antigen.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~95 kDa PSD-95 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Discs large homolog 4; Disks large homolog 4; DLG 4; Dlg4; DLG4_HUMAN; FLJ97752; FLJ98574; Human post synaptic density protein 95; Post synaptic density protein 95; Postsynaptic density protein 95; PSD 95; PSD-95; PSD95; SAP 90; SAP-90; SAP90; Synapse associated protein 90; Synapse-associated protein 90; Tax interaction protein 15

UniProt ID

P31016
UniProt Summary: Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (By similarity). Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (PubMed:26334723). {ECO:0000250|UniProtKB:Q62108, ECO:0000269|PubMed:26334723}.

NCBI Gene ID

29495

Shipping

Blue Ice

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Anti-PSD-95 Antibody

SKU: 1080-PSD95

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