Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody

Name: Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody
Brand: PhosphoSolutions
SKU: p1551-940
Price: 456.0 USD
Availability: InStock

Our Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Potassium Chloride Cotransporter (KCC2) (Ser940) and is antigen affinity purified from pooled serum. It is great for use in WB, IP.

Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Ser940 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).

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Species Reactivity

Rat

Applications

ELISA, IP, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p1551-940

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Potassium Chloride Cotransporter (KCC2) (Ser940)

Scientific Background

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser-940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IP, WB

Host Species

Rabbit

Gene Name

KCC2

Molecular Weight

135 kDa

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Non-Human Primate, Zebrafish

Antibody Registry ID

AB_2492213

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IP: 40ul of antibody per 1mg lysate

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Ser940. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser940

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Electroneutral potassium chloride cotransporter 2; Electroneutral potassium-chloride cotransporter 2; potassium chloride transporter member 5; Erythroid K Cl cotransporter 2; Furosemide sensitive K Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2; KIAA1176; Neuronal K Cl cotransporter; Neuronal K-Cl cotransporter; Potassium Chloride Cotransporter; Potassium chloride transporter 5; rKCC2; S12A5; S12A5_HUMAN; SLC12A5; Solute carrier family 12 (potassium chloride transporter member 5; Solute carrier family 12 member 5; Solute carrier family 12

UniProt ID

Q63633
UniProt Summary: Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis (PubMed:12106695). As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (By similarity). Involved in the regulation of dendritic spine formation and maturation (PubMed:24668262). {ECO:0000250|UniProtKB:Q63633, ECO:0000269|PubMed:12106695, ECO:0000269|PubMed:24668262}.

NCBI Gene ID

171373

Shipping

Blue Ice

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Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody

SKU: p1551-940

Product Details

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