Anti-NG2/CSPG4 Antibody

Name: Anti-NG2/CSPG4 Antibody
Brand: PhosphoSolutions
SKU: 1360-NG2
Price: 456.0 USD
Availability: InStock

Our NG2/CSPG4 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects NG2/CSPG4 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Immunostaining of a sagittal section of FFPE rat brain showing strong staining of oligodendrocyte precursor cells (OPC) in the hippocampus using our anti-NG2/CSPG4 (DAB, 1:200) and detected with anti-rabbit HRP. The section is counterstained with hematoxylin to identify DNA (blue).

Western blot of mouse whole brain lysate showing specific immunolabeling of the ~250 kDa CSPG4/NG2 protein.

Immunostaining of a sagittal section of FFPE rat brain showing strong staining of oligodendrocyte precursor cells (OPC) in the hippocampus using our anti-NG2/CSPG4 (DAB, 1:200) and detected with anti-rabbit HRP. The section is counterstained with hematoxylin to identify DNA (blue).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: 1360-NG2

Size: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

NG2/CSPG4

Scientific Background

Neuron-glial antigen 2 (NG2) is a chondroitin sulfate proteoglycan that is encoded by the CSPG4 gene in humans. It is an excellent oligodendrocyte marker. NG2 is a hallmark protein of oligodendrocyte progenitor cells (OPCs) and has been linked with familial schizophrenia (de Vrij, F.M., et al, 2018). NG2 also plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells (Luo, W., et al, 2006). NG2 localizes at the apical plasma membrane and relocalizes to the lamellipodia of astrocytoma upon PRKCA phosphorylation.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

CSPG4

Molecular Weight

250 kDa

Immunogen

Recombinant human NG2/CSPG4 protein

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2927667

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the recombinant protein antigen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:200

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~250 kDa NG2/CSPG4 protein

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

4732461B14Rik; AN2; melanoma-associated; AN2 proteoglycan; Chondroitin sulfate proteoglycan 4 (melanoma-associated; Chondroitin sulfate proteoglycan 4; Chondroitin sulfate proteoglycan NG2; Cspg4; Cspg4 chondroitin sulfate proteoglycan 4; CSPG4_HUMAN; HMW-MAA; HSN tumor-specific antigen; Kiaa4232; MCSP; MCSPG; MEL-CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan; MELCSPG; MSK16; NG2; Chondroitin sulfate proteoglycan 4

UniProt ID

Q6UVK1
UniProt Summary: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Also functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. {ECO:0000269|PubMed:10587647, ECO:0000269|PubMed:11278606, ECO:0000269|PubMed:15210734}.

NCBI Gene ID

1464

Shipping

Blue Ice

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Anti-NG2/CSPG4 Antibody

SKU: 1360-NG2

Product Details

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