Anti-MuRF1 (C-terminal region) Antibody

Name: Anti-MuRF1 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: MP3401
Price: 348.0 USD
Availability: InStock

Our MuRF1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat MuRF1 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).

Immunocytochemical labeling of MuRF1 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3405).

Western blot analysis of MuRF1 (Cat. MP3401), Atrogin-1 (Cat. AP2041), FoxO3a, p-AKT (Ser473), and total AKT in the soleus muscle of control, HU, and HU+TUDCA mice. Image from publication CC-BY-4.0. PMID: 37525907

Western blotting analysis of the expression of Atrogin-1 (Cat. AP2041, 1:1000) and MuRF1 (Cat. MP3401, 1:1000) proteins in the soleus muscles after HS. Image from publication CC-BY-4.0. PMID: 37344807

Western blot showing MuRF-1 (Cat. MP3401, 1:500) and atrogin-1 (Cat. AP2041), 1:500) levels in C2C12 myoblasts differentiated for 4–5 days were incubated with 200 μM UDCA for 72 h. GAPDH was used as a loading control. Image from publication CC-BY-4.0. PMID: 37237400

Markers for muscle degeneration, Myostatin and MuRF-1 (Cat. MP3401, 1:1000), were significantly less expressed in CTMP-KO gastrocnemius muscle compared to WT mice. Image from publication CC-BY-4.0. PMID: 36652767

The individual and combined effect of quercetin (Q) and low dose (LL) or high dose (HL) of leucine on relative protein expression of p-FoxO1/FoxO1, Atrogin-1 (Cat. AP2041), and MuRF1 (Cat. MP3401) in the gastrocnemius muscle in BALB/c mice exposed to cisplatin (CDDP). Image from publication CC-BY-4.0. PMID: 37699022

Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: MP3401

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

MuRF1 (C-terminal region)

Scientific Background

Muscle proteolysis is regulated by the ATP-dependent ubiquitin–proteasome system. This system involves ubiquitination of specific proteins, leading to recognition and degradation by the 26S proteasome complex. Ubiquitination requires interactions with ubiquitin related proteins, ubiquitin-activating (E1), ubiquitin-conjugating (E2) and ubiquitin-ligating enzymes (E3) known as ligases. Two muscle specific ubiquitin ligases have been identified, muscle ring finger 1 (MuRF-1) and Atrogin 1. Both ligases are regulated by the Akt1/FOXO1 signaling pathway, and both proteins have been shown to be upregulated prior to the onset of atrophy in multiple models of muscle wasting, including disuse and cachexia. MuRF1 is also known as TRIM63, SMRZ, and RNF28, and its expression is upregulated after TNFα treatment in C2C12 cells and muscle tissue, while localization of MuRF1 protein has been observed in the cytoplasm and nucleus of cells.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

TRIM63

Molecular Weight

30/38

Immunogen

MuRF1 (C-terminal) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1. This peptide sequence is highly conserved in rat and mouse MuRF1, and has 50% homology to MuRF2 (TRIM-55).

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:300

Conjugate

Unconjugated

Specificity

The antibody detects 38 and 30 kDa* proteins corresponding to the apparent molecular mass of MuRF1 isoforms on SDS-PAGE immunoblots of mouse C2C12 cells, and detects a 38 kDa band in mouse heart and muscle tissue.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

IRF; Tripartite motif 63,TRIM63; MURF2; RNF28; SMRZ; E3 ubiquitin-protein ligase TRIM63; RING-type E3 ubiquitin transferase TRIM63; iris ring finger protein; muscle specific ring finger protein 2; muscle-specific RING finger protein 1; ring finger protein 28; striated muscle RING zinc finger protein; tripartite motif-containing protein 63; Muscle RING finger; MURF-1

UniProt ID

Q969Q1
UniProt Summary: E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.

NCBI Gene ID

84676

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Anti-MuRF1 (C-terminal region) Antibody

SKU: MP3401

Product Details

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