Anti-GRIP1 Associated Protein 1 (GRASP1) Antibody

Name: Anti-GRIP1 Associated Protein 1 (GRASP1) Antibody
Brand: PhosphoSolutions
SKU: 642-GRASP
Price: 456.0 USD
Availability: InStock

Our Anti-GRIP1 Associated Protein 1 (GRASP1) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GRIP1 Associated Protein 1 (GRASP1) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of rat cerebellar lysate showing the specific immunolabeling of the ~95 kDa GRASP protein.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: 642-GRASP

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GRIP1 Associated Protein 1 (GRASP1)

Scientific Background

PDZ domain-containing proteins, such as PSD-95 and GRIP are thought to play key roles in glutamate receptor plasticity. GRIP-associated proteins (GRASPs) that bind to distinct PDZ domains within GRIP also play key roles in regulation of glutamate receptor function. GRASP-1 is a neuronal rasGEF associated with GRIP and AMPA receptors in vivo (Scannevin and Huganir, 2000). Recent work suggests that GRASP-1 may regulate neuronal ras signaling and contribute to the regulation of AMPA receptor distribution by NMDA receptor activity (Ye et al., 2000).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

GRIPAP1

Molecular Weight

95 kDa

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat GRASP1, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Human

Antibody Registry ID

AB_2492135

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~95 kDa GRASP1 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

DKFZp434P0630; DXImx47e; GRAP1_HUMAN; GRASP 1; GRASP-1; GRASP1; GRIP 1 associated protein 1; GRIP associated protein 1; GRIP1 associated protein 1; GRIP1-associated protein 1; GRIPAP 1; GRIPAP1; KIAA1167; MGC126593; MGC126595; MPMGp800B12492Q3; Sfc10; TAMALIN

UniProt ID

Q9JHZ4
UniProt Summary: Regulates the endosomal recycling back to the neuronal plasma membrane, possibly by connecting early and late recycling endosomal domains and promoting segregation of recycling endosomes from early endosomal membranes. Involved in the localization of recycling endosomes to dendritic spines, thereby playing a role in the maintenance of dendritic spine morphology. Required for the activity-induced AMPA receptor recycling to dendrite membranes and for long-term potentiation and synaptic plasticity (By similarity). {ECO:0000250|UniProtKB:Q9JHZ4}.; [GRASP-1 C-terminal chain]: Functions as a scaffold protein to facilitate MAP3K1/MEKK1-mediated activation of the JNK1 kinase by phosphorylation, possibly by bringing MAP3K1/MEKK1 and JNK1 in close proximity. {ECO:0000269|PubMed:17761173}.

NCBI Gene ID

116493

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Scannevin RH, Huganir RL (2000) Postsynaptic organization and regulation of excitatory synapses. Nat Rev Neurosci 1:133-141. PMID: 11252776
Ye B, Liao DZ, Zhang XQ, Zhang PS, Dong HL, Huganir RL (2000) GRASP-1: A neuronal RasGEF associated with the AMPA receptor/GRIP complex. Neuron 26:603-617. PMID: 10896157

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Anti-GRIP1 Associated Protein 1 (GRASP1) Antibody

SKU: 642-GRASP

Product Details

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