Our Anti-Dopamine Transporter, C-Terminus rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, non-human primate, and rat Dopamine Transporter, C-Terminus and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.
The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, IHC, WB
Rabbit
SLC6A3
80 kDa
Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region of human dopamine transporter, conjugated to keyhole limpet hemocyanin (KLH).
Human
Human, Mouse, Non-Human Primate, Rat
AB_2492076
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification using a column matrix to which the peptide immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:1000
Unconjugated
Specific for endogenous levels of the ~80 kDa DAT protein. Diffuse band may be observed between ~70-85 kDa due to variable glycosylation.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
DA transporter; DAT 1; neurotransmitter transporter dopamine member 3; DAT; neurotransmitter transporter member 3; DAT1; VNTR; Dopamine transporter 1; Dopamine transporter; PKDYS; SC6A3_HUMAN; SLC6A3; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine member 3; Solute carrier family 6 (neurotransmitter transporter member 3; Solute carrier family 6 member 3; Variable number tandem repeat (VNTR; Solute carrier family 6; Variable number tandem repeat
Q01959
UniProt Summary: Mediates sodium- and chloride-dependent transport of dopamine (PubMed:10375632, PubMed:11093780, PubMed:1406597, PubMed:15505207, PubMed:19478460, PubMed:39112701, PubMed:39112703, PubMed:39112705, PubMed:8302271). Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (By similarity). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity). {ECO:0000250|UniProtKB:P23977, ECO:0000250|UniProtKB:Q61327, ECO:0000269|PubMed:10375632, ECO:0000269|PubMed:11093780, ECO:0000269|PubMed:1406597, ECO:0000269|PubMed:15505207, ECO:0000269|PubMed:19478460, ECO:0000269|PubMed:39112701, ECO:0000269|PubMed:39112703, ECO:0000269|PubMed:39112705, ECO:0000269|PubMed:8302271}.
Trial Size
Pooled Serum