Anti-Bad (Ser-26), Phosphospecific Antibody

Name: Anti-Bad (Ser-26), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: BP4951
Price: 348.0 USD
Availability: InStock

Our Bad (Ser-26) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Bad (Ser-26) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-26) (lanes 3 & 4).

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Species Reactivity

Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: BP4951

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Bad (Ser-26)

Scientific Background

Bad is a member of the BCL-2 family of regulators involved in programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT IKK, and MAP kinases, as well as protein phosphatase calcineurin are found to be involved in the regulation of this Bad activity. Phosphorylation of Bad occurs on one or more of Ser-26, Ser-112, Ser-136, and Ser-155 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-136 or Ser-112 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-155, a site within the BH3 motif, leading to the release of Bcl-xL and the promotion of cell survival. Ser-26 is phosphorylated by IKK leading to phosphorylation of C-terminal serine sites and disruption of binding to Bcl-xL. This inactivation of Bad inhibits TNFα-induced apoptosis independent of NF-κB activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

BAD

Molecular Weight

Immunogen

Phospho-Bad (Ser-26) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 26 in mouse Bad. This peptide sequence is highly conserved in rat Bad, but is not found in human Bad.

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

The antibody detects a 23 kDa* doublet corresponding to the apparent molecular mass of phosphorylated Bad on SDS-PAGE immunoblots of mouse J774A.1 treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-26

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

AI325008; Bcl2 antagonist of cell death; bcl2-associated agonist of cell death; Bcl-2-binding component 6; bcl-xL/Bcl-2-associated death promoter; BAD; Bbc6

UniProt ID

Q61337
UniProt Summary: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

NCBI Gene ID

12015

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Anti-Bad (Ser-26), Phosphospecific Antibody

SKU: BP4951

Product Details

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