Anti-ALDH1A1 (N-terminal region) Antibody (M562)

Name: Anti-ALDH1A1 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: AM5621
Price: 379.0 USD
Availability: InStock

Our ALDH1A1 (N-terminal region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human ALDH1A1 (N-terminal region) and is Protein G purified. It is great for use in WB, ICC.

Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human ALDH1A1 (lane 6). The blot was probed with mouse monoclonal ALDH1A1 M562 (lanes 1-6) at a dilution of 1:1,000.

Immunocytochemical labeling of ALDH1A1 in aldehyde fixed and NP-40 permeabilized human A431 cells. The cells were labeled with mouse monoclonal anti-ALDH1A1 (AM5621). The antibody was detected using goat anti-mouse DyLight® 594.

Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human ALDH1A1 (lane 6). The blot was probed with mouse monoclonal ALDH1A1 M562 (lanes 1-6) at a dilution of 1:1,000.

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, ICC, WB

Host Species

Mouse

Go to Full Product Details

Data Sheet

SKU: AM5621

Volume: 100 µL

Variant

Price:

$379.00

Quantity:

Ships: 1-2 business days

Product Details

Target

ALDH1A1 (N-terminal region)

Scientific Background

Aldehyde dehydrogenase (ALDH) superfamily is a ubiquitous group of enzymes found in all taxonomic domains. ALDH detoxifies endogenous and exogenous aldehydes, protecting cellular homeostasis and organismal functions. These enzymes are necessary for the synthesis of retinoic acid, betaine, and folate. Recent studies have reported high levels of ALDH found in cancer cells, suggesting that ALDH can act as a marker for cancer cells found in a wide variety of tissues including skin, prostate, lung, and neural tissues. Additionally, certain diseases can be identified when ALDH activity is absent. ALDH1A1 is vital for retinol synthesis and alcohol metabolism. ALDH1A1 active sites include an active cysteine residue, which catalyses the transformation of aldehydes into their respective carboxylic groups. ALDH1A1 amino acid sequence and function is highly conserved in humans and rodents.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M562

Isotype

IgG2a

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

ALDH1A1

Molecular Weight

Immunogen

Clone M562 was generated from a recombinant protein corresponding to amino acids in the N-terminal region from human ALDH1A1.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody antibody detects a 55 kDa* protein on SDS-PAGE immunoblots of human A431, HepG2, and mouse liver, as well as human recombinant ALDH1A1. The antibody does not detect ALDH1A3 in PC3 cells, ALDH1A2 in Jurkat cells, and bovine tubulin (55 kDa).

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

ALDH1; RALDH; ALDH11; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1; retinal dehydrogenase 1; ALDH class 1; ALHDII; RALDH 1; acetaldehyde dehydrogenase 1; aldehyde dehydrogenase 1; soluble; aldehyde dehydrogenase; liver cytosolic; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; retinaldehyde dehydrogenase 1; ALDC; ALDH-E1; ALDH1A1

UniProt ID

P00352
UniProt Summary: Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid. Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid. This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic. Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification. Also functions downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins. Also has an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission.

NCBI Gene ID

216

Shipping

Blue Ice

Recover password

Create my account

Anti-ALDH1A1 (N-terminal region) Antibody (M562)

SKU: AM5621

Product Details

Bulk Order Anti-ALDH1A1 (N-terminal region) Antibody

Related Products

Recently Viewed