Anti-α-Actinin 4 (N-terminal) Antibody

Name: Anti-α-Actinin 4 (N-terminal) Antibody
Brand: PhosphoSolutions
SKU: AP4271
Price: 292.0 USD
Availability: InStock

Our α-Actinin 4 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat α-Actinin 4 and is antigen affinity purified. It is great for use in WB.

Western blot analysis of α-actinin in human A431 cells (lanes 1 & 3) and rabbit spleen fibroblasts (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-α-actinin 4 (a.a. 2-11) or mouse monoclonal anti-α-actinin (C-terminal region).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: AP4271

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

α-Actinin 4

Scientific Background

α-Actinins are widely expressed cytoskeletal proteins that cross-link actin filaments through anti-parallel homodimers of the rod domains. Four α-actinin genes have been discovered in humans with α-actinin 1 and 4 being widely expressed in non-muscle cells. α-Actinins contain three conserved domains that include an N-terminal actin binding domain, four spectrin-like repeats in the central region, and a C-terminal calmodulin binding domain. α-Actinin cross-links the actin filament networks and associates the network to focal adhesion sites through binding of talin and vinculin. α-Actinin 1 is phosphorylated at Tyr-12 by FAK, while α-actinin 4 can be phosphorylated at Tyr-4 and Tyr-31 after EGF treatment. Tyr-4 and Tyr-31 phosphorylation inhibit actin binding and reduces actin-filament driven multi-nucleation in rat kidney cells. Thus, phosphorylation in α-actinins may be important for regulating actin binding and actin cytoskeletal remodeling.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

ACTN4

Molecular Weight

100

Immunogen

α-Actinin 4 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminus of human α-actinin 4. This sequence is well conserved in rat and mouse α-actinin 4, but is not conserved in other α-actinins.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

The antibody detects a 100 kDa* protein corresponding to the molecular mass of α-actinin 4 on SDS-PAGE immunoblots of human A431 cells and rabbit spleen fibroblasts.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

actinin 4; a-actinin 4; Actinin alpha 4; actinin4; ACTN 4; ACTN4; ACTN4_HUMAN; alpha Actinin 4; Alpha-actinin-4; DKFZp686K23158; F actin cross linking protein; F-actin cross-linking protein; Focal segmental glomerulosclerosis 1; FSGS 1; FSGS; FSGS1; Non muscle alpha actinin 4; Non-muscle alpha-actinin 4; actinin alpha4

UniProt ID

O43707
UniProt Summary: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation.

NCBI Gene ID

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Anti-α-Actinin 4 (N-terminal) Antibody

SKU: AP4271

Product Details

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