Our Anti-14-3-3 Protein rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat 14-3-3 Protein and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein.
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, WB
Rabbit
YWHAB
29 kDa
Peptide corresponding to amino acid residues from the N-terminal region of the rat 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol.
Liquid
Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol
WB: 1:1000
WB Brain: 1:1000
IHC: Not recommended for use in paraffin sections
Unconjugated
Specific for endogenous levels of the ~29 kDa 14-3-3 protein. Immunolabeling is blocked by preadsorption of the antibody with the peptide immunogen.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
14 3 3; 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta ,14 3 3 zeta ,14-3-3 protein beta/alpha; 14-3-3 protein/cytosolic phospholipase A2; 1433B_HUMAN; GW128; HS1; KCIP 1; KCIP-1; MGC111427; MGC126532; MGC138156; N-terminally processed; Protein 1054; Protein kinase C inhibitor protein 1; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein delta polypeptide; Tyrosine 3/tryptophan 5 -monooxygenase activation protein zeta polypeptide; YWHAB; YWHAD; YWHAZ
P35213
UniProt Summary: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13. {ECO:0000269|PubMed:17717073, ECO:0000269|PubMed:19592491, ECO:0000269|PubMed:21224381}.
