Anti-GluN2A/NR2A Glutamate Receptor Recombinant Chicken Chimeric mAb (N327/95)

Name: Anti-GluN2A/NR2A Glutamate Receptor Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-288
Price: 481.0 USD
Availability: InStock

Our chicken Anti-GluN2A/NR2A glutamate receptor recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N327/95. It detects human, mouse, rabbit, and rat GluN2A/NR2A is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum showing cells stained by anti-GluN2A/NR2A Glutamate Receptor recombinant chicken mAb (green, Cat No. 78-288) using goat anti-chicken IgY CF®488 secondary antibody (Cat No. 80-1001-488). The stained sections were mounted with Antibodies Incorporated’s Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

$Western blotting of rat brain membrane with anti-GluN2A/NR2A Glutamate Receptor recombinant chicken mAb (Cat No. 78-288). The chicken mAb was detected with anti-chicken-HRP (Cat No. 80-1000-HRP) and recognizes endogenous NR2A in the rat brain membrane fraction at ~170 kDa.$

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus showing cells stained by anti-GluN2A/NR2A Glutamate Receptor recombinant chicken mAb (green, Cat No. 78-288) using goat anti-chicken IgY CF®488 secondary antibody (Cat No. 80-1001-488).

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum showing cells stained by anti-GluN2A/NR2A Glutamate Receptor recombinant chicken mAb (green, Cat No. 78-288) using goat anti-chicken IgY CF®488 secondary antibody (Cat No. 80-1001-488). The stained sections were mounted with Antibodies Incorporated’s Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

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Species Reactivity

Human, Mouse, Rabbit, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-288

Volume: 100 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GluN2A/NR2A glutamate receptor

Scientific Background

Glutamate receptor NMDA2A or GluN2A/NR2A glutamate receptor is a member of the glutamate-gated ion channel family. NR2A is a subunit of the ligand-gated ion channel with high calcium permeability and voltage-dependent sensitivity to magnesium. NR2A is expressed throughout the brain, including striatum, forbrain, cerebellum (at protein level) brain cortex, piriform cortex, hippocampus, caudate-putamen, dentate gyrus and granule cell layer. It is found at the postsynaptic cell membrane, dendritic spine, synapse, and cytoplasmic vesicle membrane. A variety of neurological diseases, including epilepsy, intellectual disability, autism spectrum disorders, developmental delay, and schizophrenia are associated with mutations in the NR2A gene.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N327/95

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Grin2a

Molecular Weight

170 kDa

Excitation / Emission

Unconjugated

Immunogen

Fusion protein amino acids 75-325 (extracellular N-terminus) of rat NR2A (accession number Q00959) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rabbit, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N327/95 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N327/95 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:500-1:1000
WB Brain: 1:500-1:1000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

Does not cross-react with GluN2B/NR2B

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Glutamate receptor ionotropic, NMDA 2A (GluN2A) (Glutamate [NMDA] receptor subunit epsilon-1) (N-methyl D-aspartate receptor subtype 2A) (NMDAR2A) (NR2A)

UniProt ID

Q00959

Shipping

Shipped on ice packs

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Anti-GluN2A/NR2A Glutamate Receptor Recombinant Chicken Chimeric mAb (N327/95)

SKU: 78-288

Product Details

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