Phalloidin Conjugated with Tetramethylrhodamine (TRITC)

Name: Phalloidin Conjugated with Tetramethylrhodamine (TRITC)
Brand: PhosphoSolutions
SKU: PF7551
Price: 165.0 USD
Availability: InStock

1 Citation

Phalloidin conjugated to TRITC via the side chain of amino acid 7.

Fluorescent labeling of actin filaments in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with phalloidin:TRITC (PF7551) at a dilution of 1:2000.

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Species Reactivity

Chicken, Fish, Human, Mouse, Rat

Applications

ICC, IHC

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Data Sheet

SKU: PF7551

Volume: 100 µL

Variant

Price:

$165.00

Quantity:

Ships: 1-2 business days

Product Details

Scientific Background

Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F-actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. Fluorescent conjugates of phalloidin are commonly used to label actin filaments for immunocytochemical and immunohistochemical applications. Both rhodamine and fluorescein labeled phalloidin can be used to localize and quantify actin filaments in cells and tissues.

Applications

ICC, IHC

Molecular Weight

1300 kDa

Excitation / Emission

TRITC Ex: 540 nm, Em: 570 nm

Species Reactivity

Chicken, Fish, Human, Mouse, Rat

Storage

Store at –20°C. Stable for 1 year.

Physical State

Liquid

Buffer

Phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Dilution Ranges

IHC: 1:1000
ICC: 1:1000

Conjugate

FITC

Specificity

Phalloidin:TRITC can be used for immunocytochemical and immunohistochemical labeling of actin filaments. Phalloidin: TRITC binds polymeric and oligomeric actin, but does not bind monomeric actin. The conjugates can be used to label actin filaments in many species, including human, mouse, rat, chicken, and fish.

Country of Origin

U.S.A.

Expiration

Stable for 1 year after date of receipt.

Shipping

Blue Ice

Western Blot Protocol: Download

Product Specific References for Applications and Species

Immunocytochemistry

Immunocytochemistry
PMID	Dilution	Publication
39796153	not listed	Klabenkova, K, et al. 2024. Synthesis of New Polyfluoro Oligonucleotides via Staudinger Reaction. International Journal of Molecular Sciences, 300-300.

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Phalloidin Conjugated with Tetramethylrhodamine (TRITC)

SKU: PF7551

Product Details

Product Specific References for Applications and Species

Bulk Order Phalloidin Conjugated with Tetramethylrhodamine (TRITC)

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