Primary Antibodies

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Immunofluorescence staining transiently Kv1.6-transfected COS cells.Immunoblot on adult rat brain membrane (RBM) and lysates from Kv1.6-transfected (COS+) and untransfected (COS-) COS cells.
Immunolabeling of postnatal 14 mouse hippocampus specifically labeling Kv1.1 (Cat. 75-007, 1:200, green) and Ankyrin G (red). DNA labeled with DAPI. Mice were transcardially perfused with ice-cold 4% paraformaldehyde in PBS, then brain removed and postfixed overnight. The 50 uM slices were floating sections, where antigen retrieval was performed with sodium citrate at 80 C for 30 minutes before immunolabeling. Images from J. Ramirez-Franco and O. El Far, Aix-Marseille Université and INSERM.Adult rat brain hippocampus immunohistochemistry.
Adult rat brain membrane immunoblot.Immunoblot on adult mouse brain membranes from two wild-type (WT1,2) and Kvβ2 knockout (Kvβ2 KO) mice. Brain samples courtesy of Dr. Geoff Murphy, Molecular and Behavioral Neuroscience Institute, University of Michigan.
Adult rat brain membrane immunoblot.Immunoblot on membrane fractions of whole rat (RBM) and mouse (MBM) brain and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Immunoblot versus crude membranes from adult rat brain (RBM) and wild-type (WT) and LRRK2 KO mouse brains probed with 8G10 (left), N241A/34 (middle) and K89/34 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson, Johns Hopkins University
Adult rat brain membrane immunoblotAdult rat whole brain immunohistochemistry
Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Adult rat brain membrane immunoblot
"Endogenous and transfected cell immunoblot: extracts of HEK-293 cells transfected with GFP-tagged LRRK2-WT, LRRK2-S910A or LRRK2-S935A plasmid; fibroblasts from embryonic WT or LRRK2 KO mice untreated or treated with LRRK2-IN-1 inhibitor (2011 Deng et al Nat Chem Biol); and human lymphoblasts untreated or treated with LRRK2-IN-1, probed with 1D8. Data courtesy of Nicolas Dzamko, Paul Davies and Dario Alessi (University of Dundee, Scotland, UK)."

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