Anti-WAVE2 (Tyr-150) [conserved site], Phosphospecific Antibody

Name: Anti-WAVE2 (Tyr-150) [conserved site], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: WP1821
Price: 348.0 USD
Availability: InStock

Our WAVE2 (Tyr-150) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat WAVE2 (Tyr-150) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot of human K562 cells treated with pervanadate (1 mM, 30 min) (lanes 1 & 3) then treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with anti-WAVE2 (Central region) (lanes 1 & 2) or anti-WAVE (Tyr-150) (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

Go to Full Product Details

Data Sheet

SKU: WP1821

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

WAVE2 (Tyr-150)

Scientific Background

The Wiskott–Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVE1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

WASF2

Molecular Weight

Immunogen

Phospho-WAVE (Tyr-150) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-150 in human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and has homology to the conserved sites in WAVE1 (Tyr-151) and WAVE3 (Tyr-151).

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-WAVE (Tyr-125) and unphosphorylated WAVE (Tyr-150) peptides before affinity purification using phospho-WAVE (Tyr-150) peptide. In western blots, the antibody detects a 78 kDa* corresponding to phosphorylated WAVE2 in K562 cells treated with pervanadate and a 80 kDa band corresponding to WAVE1 in HUVEC treated with pervanadate. It also recognizes an unidentified band near 50 kDa in K562 cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-150

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Wiskott-Aldrich syndrome verproline, Scar2, WASF2

UniProt ID

Q9Y6W5

Shipping

Blue Ice

Recover password

Create my account

Anti-WAVE2 (Tyr-150) [conserved site], Phosphospecific Antibody

SKU: WP1821

Product Details

Bulk Order Anti-WAVE2 (Tyr-150) [conserved site], Phosphospecific Antibody

Related Products

Recently Viewed