Anti-VGAT Recombinant Chicken Chimeric mAb (BSA and Azide Free) (L118/80)

Name: Anti-VGAT Recombinant Chicken Chimeric mAb (BSA and Azide Free)
Brand: Aves Labs
SKU: 78-457-CF
Price: 522.0 USD
Availability: InStock

Our chicken Anti-VGAT carrier-free recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L118/80. It detects mouse and rat VGAT and is purified by affinity chromatography. Works in WB, IHC, ICC, ELISA.

Anti-VGAT Recombinant Chicken Chimeric mAb (BSA and Azide Free)

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Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Biotin

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Data Sheet

SKU: 78-457-CF

Volume: 100 µL

Variant

Price:

$522.00

Quantity:

Ships: 1-5 business days

Product Details

Target

VGAT

Scientific Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.6-1.0 mg/mL

Clonality

Recombinant

Clone

L118/80

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Slc32a1 Vgat Viaat

Molecular Weight

50 kDa

Excitation / Emission

Unconjugated

Immunogen

Fusion protein amino acids 1-133 (cytoplasmic N-terminus) of mouse VGAT (accession number O35633) produced recombinantly in E. Coli

Immunogen Species

Mouse

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L118/80 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L118/80 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

Buffer

PBS

Dilution Ranges

WB: 1:500-1:1000
IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated, Carrier Free

Specificity

No cross-reactivity reported

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Vesicular inhibitory amino acid transporter (GABA and glycine transporter) (Solute carrier family 32 member 1) (Vesicular GABA transporter) (mVGAT) (mVIAAT)

UniProt ID

O35633

Shipping

Shipped on ice packs

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Anti-VGAT Recombinant Chicken Chimeric mAb (BSA and Azide Free) (L118/80)

SKU: 78-457-CF

Product Details

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