Our Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Vesicular Monoamine Transporter 2, C-terminus (VMAT2) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat striatal lysate showing specific immunolabeling of the ~57 kDa VMAT2 protein.
Vesicular neurotransmitter transporters sequester the transmitters into synaptic vesicles (Erickson et al., 1996). The vesicular monoamine transporter 2 (VMAT2) is responsible for catecholamine and serotonin storage in central synapses. Antibodies specific for VMAT have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, IHC, WB
Sheep
VMAT2
57 kDa
Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region of human VMAT2, conjugated to keyhole limpet hemocyanin (KLH).
Human
Human, Rat
AB_2492286
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled sheep serum by affinity purification using a column to which the peptide immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:200
Unconjugated
Specific for endogenous levels of the ~57 kDa VMAT2 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
