Our Anti-Vesicular GABA transporter (VGAT) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, mouse, rat, and Xenopus Vesicular GABA transporter (VGAT) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.
Western blot of rat olfactory bulb lysate showing specific immunolabeling of the ~53 kDa VGAT protein.
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, ICC, IHC, WB
Rabbit
SLC32A1
53 kDa
Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat VGAT, conjugated to keyhole limpet hemocyanin (KLH).
Rat
Chicken, Mouse, Rat
Bovine, Canine, Non-Human Primate
AB_2492282
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification on a column made with the peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Specific for endogenous levels of the ~53 kDa VGAT protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
