Anti-Vav2 Antibody

Name: Anti-Vav2 Antibody
Brand: PhosphoSolutions
SKU: VP2481
Price: 292.0 USD
Availability: InStock

Our Vav2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Vav2 and is antigen affinity purified. It is great for use in WB, ICC, IP.

Western blot of human A431 cells treated with EGF (lanes 1, 3, & 5) then the blot was exposed to alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Vav (a.a. 165-174) (lanes 1 & 2), anti-Vav2 (Tyr-142) (lanes 3 & 4), or anti-Vav2 (Tyr-172) (lanes 5 & 6).

Immunocytochemical labeling of Vav in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Vav (a.a. 165-174), and detected using appropriate secondary antibody conjugated to Cy3.

Western blot of human A431 cells treated with EGF (lanes 1, 3, & 5) then the blot was exposed to alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Vav (a.a. 165-174) (lanes 1 & 2), anti-Vav2 (Tyr-142) (lanes 3 & 4), or anti-Vav2 (Tyr-172) (lanes 5 & 6).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IP, WB

Host Species

Rabbit

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Data Sheet

SKU: VP2481

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Vav2

Scientific Background

The Vav family of Rho-guanine nucleotide exchange factors, Vav1, Vav2, and Vav3, have central roles in transducing signals from cell surface receptors, such as integrin, growth factor and immune cell receptors to the cytoskeleton. This role includes receptor-mediated changes in the actin cytoskeleton and cell motility. Vav1 expression is normally restricted to hematopoietic cells, while Vav2 and Vav3 are more widely expressed. All three Vav isoforms have been shown to be abnormally expressed in several types of cancer. Vavs are composed of multiple domains, including a Dbl homology domain, a calponin homology domain, an acidic amino acid region, a pleckstrin homology domain, a cysteine-rich domain, and SH3 and SH2 domains. Vav activity is regulated by the phosphorylation status of several conserved tyrosine residues in the acidic region (In Vav2: Tyr-142, Tyr-159, and Tyr-172). These tyrosine residues are able to participate in autoinhibitory interactions with the Dbl homology domain and this interaction is prevented after phosphorylation of these sites leading to activation of Vav GEF activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IP, WB

Host Species

Rabbit

Gene Name

VAV2

Molecular Weight

100

Immunogen

Vav2 synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-172 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3.

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 100 kDa* band corresponding to Vav2 on SDS-PAGE immunoblots of human A431 cells and detects several Vav isoforms in Jurkat cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

VAV2, Guanine nucleotide exchange factor VAV2

UniProt ID

Q60992

Shipping

Blue Ice

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Anti-Vav2 Antibody

SKU: VP2481

Product Details

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