Anti-Vanilloid receptor-like protein 1 (VRL-1) Antibody

Name: Anti-Vanilloid receptor-like protein 1 (VRL-1) Antibody
Brand: Biosensis
SKU: R-078-50
Price: 327.0 USD
Availability: InStock

Our Anti-Vanilloid receptor-like protein 1 (VRL-1) rabbit polyclonal primary antibody detects human and rat Vanilloid receptor-like protein 1 (VRL-1), and is affinity-purified. It is validated for use in IHC-Frozen, WB.

Immunohistochemical staining of Vanilloid Receptor Like Protein (VRL-1) in rat trigeminal ganglion using rabbit polyclonal antibody to rat VRL-1, catalogue number R-078-50.

Western blot of VRL-1 in PC12 cell lysate (RIPA, 80 µg/lane). R-078-50 detects glycosylated VRL-1 monomer at around 90-95 kDa. VRL-1 dimer is also detected by this antibody in this cell lysate preparation. SDS-PAGE: denatured and non- reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 µm); Blocking: 5% skim milk in TBST, 2 hours at RT; Primary antibody: overnight at 4°C (2 µg/mL); Secondary antibody: anti-rabbit-HRP (1/6000), 1 hour at RT; Detection: Chemiluminiscence

Western blot analysis of VRL-1 expression in rat cell lysate and tissue homogenates (RIPA extracts). One prominent band at ~90 kDa is observed corresponding to the expected molecular weight of VRL-1 protein. SDS-PAGE: denatured and reduced; Transfer: Towbin's buffer (20% MeOH, no SDS); Membrane: PVDF (0.45 um); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: overnight at 2-8°C (1 µg/mL); Secondary antibody: anti-rabbit-HRP (1/10,000), 1 hours at RT; Detection: Chemiluminiscence.

Immunohistochemical staining of Vanilloid Receptor Like Protein (VRL-1) in rat trigeminal ganglion using rabbit polyclonal antibody to rat VRL-1, catalogue number R-078-50.

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Species Reactivity

Human, Rat

Applications

IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-078-50

Volume/Size: 50 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Vanilloid receptor-like protein 1 (VRL-1)

Scientific Background

TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Immunogen

A synthetic peptide (C-KNSASEEDHLPLQVLQSP) as part of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen.

Immunogen Species

Rat

Species Reactivity

Human, Rat

Storage

Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.

Physical State

Lyophilized

Buffer

Lyophilized from PBS, pH 7.4, containing 0.02% sodium azide as preservative.

Dilution Ranges

WB: 0.5-1 µg/mL
IHC: 0.5-1 µg/mL
ICC: 0.5-1 µg/mL

Application Details

This antiserum works for immunohistochemistry on free-floating or paraffin embedded sections.

Conjugate

Unconjugated

Specificity

Immunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot. This antibody is known to react with rat and human VRL-1.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

osm-9-like TRP channel 2; OTRPC2

UniProt ID

Q9WUD2

Shipping

25°C (ambient)

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Anti-Vanilloid receptor-like protein 1 (VRL-1) Antibody

SKU: R-078-50

Product Details

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