Anti-VAChT Recombinant Chicken Chimeric mAb (N425/45)

Name: Anti-VAChT Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-429
Price: 481.0 USD
Availability: InStock

Our chicken Anti-VAChT recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N425/45. It detects mouse and rat VAChT and is purified by affinity chromatography. Works in WB, IHC, ICC, ELISA.

Representative section of formalin fixed, paraffin-embedded rat brain stem showing facial motor neurons stained by VAChT (Slc18a3) recombinant chicken monoclonal antibody (Cat. 78-180) using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

Western blotting of mock transfected and VAChT transfected COS-7 lysates with anti-VAChT (Slc18a3) mouse NeuroMab (Cat. 75-429) and Aves Labs anti-VAChT recombinant chicken mAb (Cat. 78-429) antibody. The mouse mAb and chicken mAb were detected with anti-mouse-HRP and anti-chicken-HRP (Cat. 80-1000-HRP), respectively. Aves Labs Recombinant Chicken anti-VAChT mAb recognizes exogenous VAChT with similar sensitivity as the anti-VAChT NeuroMab only in VAChT transfected COS-7 lysates at ~60 kDa.

Representative section of formalin fixed, paraffin-embedded rat brain stem showing facial motor neurons stained by VAChT (Slc18a3) recombinant chicken monoclonal antibody (Cat. 78-180) using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

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Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-429

Volume: 100 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VAChT

Scientific Background

Vesicular acetylcholine transporter (VaCht), or Solute Carrier Family 18 Member A3 is encoded by the gene Slc18a3 and is a member of the vesicular amine transporter family. VaCht transports acetylcholine into secretory vesicles for release into the extracellular space. VaCht is expressed in major cholinergic cell groups in the peripheral and central cholinergic nervous systems. Diseases associated with SLC18A3 include Myasthenic Syndrome and Presynaptic and Presynaptic Congenital Myasthenic Syndromes.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N425/45

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Slc18a3 Vacht Vat

Molecular Weight

60 kDa

Immunogen

Fusion protein amino acids 55-125 (first vesicle lumen domain) of rat VAChT (accession number Q62666) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N425/45 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N425/45 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

Does not cross-react with VMAT1 or VMAT2

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Vesicular acetylcholine transporter (VAChT) (rVAT) (Solute carrier family 18 member 3)

UniProt ID

Q62666

Shipping

Shipped on ice packs

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Anti-VAChT Recombinant Chicken Chimeric mAb (N425/45)

SKU: 78-429

Product Details

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