Anti-Tyrosine Hydroxylase Recombinant Chicken Chimeric mAb FL594 Conjugate (LNC1)

Name: Anti-Tyrosine Hydroxylase Recombinant Chicken Chimeric mAb FL594 Conjugate
Brand: Aves Labs
SKU: 78-700-FL594
Price: 562.0 USD
Availability: InStock

Our chicken Anti-Tyrosine Hydroxylase (TH) FL594 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone LNC1. It detects chicken, frog, human, lizard, monkey, mouse, rat, vole, and zebrafish Tyrosine Hydroxylase (TH) and is purified by affinity chromatography. Works in ICC, IHC.

Anti-Tyrosine Hydroxylase Recombinant Chicken Chimeric mAb FL594 Conjugate

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Applications

ICC, IHC

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

650

Biotin

Carrier-Free

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Data Sheet

SKU: 78-700-FL594

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Tyrosine Hydroxylase (TH)

Scientific Background

TH is the rate-limiting enzyme in the synthesis of the catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine and is responsible for converting L-tyrosine to L-dopa. Synthesis of catecholamines is regulated by the interaction of TH with its’ cofactor, tetrahydrobiopterin (BH4) and the substrates L-tyrosine and molecular oxygen. In humans four TH mRNA splice variants (hTH1-hTH4) have been isolated while subprimate species rely on a single form of TH. It is known that the hTH1-hTH4 variants are identical in their catalytic domain but differ in their N-terminal regulatory domains. Importantly, LNC1 reacts with the catalytic domain of TH and thus with all four isoforms of human TH. The role of TH in the synthesis of catecholamine neurotransmitters suggests a connection between the enzyme and a number of neuropathogenic diseases characterized by irregular catecholamine levels, such as Parkinson's disease, schizophrenia, and dystonia, as well as a variety of cardiovascular diseases.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

LNC1

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

TH TYH EC 1.14.16.2

Molecular Weight

59-63 kDa

Excitation / Emission

FL594 Ex: 594 nm, Em: 615 nm

Immunogen

Tyrosine Hydroxylase purified from PC12 cells

Immunogen Species

Human

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone LNC1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone LNC1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Conjugate

594

Specificity

Recognizes an epitope outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: purified dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT). Identifies a single ~60 kDa band on Western Blots of HeLa cells transfected to express human tyrosine hydroxylase.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Tyrosine 3-monooxygenase

UniProt ID

P07101

Shipping

Shipped on ice packs

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Anti-Tyrosine Hydroxylase Recombinant Chicken Chimeric mAb FL594 Conjugate (LNC1)

SKU: 78-700-FL594

Product Details

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