Anti-TRIP8b (Constant) Antibody (N212/17)

Name: Anti-TRIP8b (Constant) Antibody
Brand: NeuroMab
SKU: 75-244
Price: 337.0 USD
Availability: InStock

Our mouse TRIP8b (constant) monoclonal antibody from NeuroMab is produced in-house from clone N212/17. It detects human, mouse, and rat TRIP8b (constant) and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b- 1b-2, TRIP8b-1a-2 and untagged Kv2.1 plasmids and probed with N212/17 (left) and N86/8 (right) TC supe.

Immunohistochemistry of the cell body layer of CA1 of Trip8b+/+ and Trip8b–/– mice showing specific lableling of HCN2, Myelin Basic Protein, and TRIP8b (Cat. 75-244, 1:1000). Image from publication CC-BY-4.0. PMID:38469353

Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-244

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

TRIP8b (constant)

Scientific Background

PEX5-related protein, Peroxisomal Biogenesis Factor 5 Like, TPR-containing Rab8b-interacting protein or TRIP8b is encoded by the gene Pex5l. It is a member of the peroxisomal targeting signal receptor family. TRIP8b is a cytoplasmic accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts with the carboxyl-terminal region of HCN channels and regulates their cell-surface expression to the dendrites in many types of neurons. TRIP8b plays a role in regulation of HCN channel cyclic nucleotide kinetics. TRIP8b is expressed in the brain. Diseases associated with PEX5L include Rhizomelic Chondrodysplasia Punctata, Type 5 and Rhizomelic Chondrodysplasia Punctata, Type 2.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N212/17

Isotype

IgG2a

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Pex5l Pex2 Pex5r Trip8b

Molecular Weight

70 kDa

Immunogen

Fusion protein amino acids 1-192 (exon 1a, exon 4 and constant region) of rat TRIP8b (accession number Q925N3) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10698035

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Cross-reacts with multiple TRIP8b isoforms

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

PEX5-related protein (PEX5-like protein) (Peroxin-5-related protein) (TPR-containing Rab8b-interacting protein) (Tetratricopeptide repeat-containing Rab8b-interacting protein) (Pex5Rp) (TRIP8b)

UniProt ID

Q925N3

Shipping

Shipped on ice packs

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Anti-TRIP8b (Constant) Antibody (N212/17)

SKU: 75-244

Product Details

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