Anti-Sphingosine Kinase 1 (Ser-225), Phosphospecific Antibody

Name: Anti-Sphingosine Kinase 1 (Ser-225), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: SP1641
Price: 348.0 USD
Availability: InStock

Our Sphingosine Kinase 1 (Ser-225) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Sphingosine Kinase 1 (Ser-225) and is antigen affinity purified. It is great for use in WB.

Western blot image of recombinant his-tagged human SK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-SK1 (Central Region) (SP1621; lanes 1-3) and anti-SK1 (Ser-225) (SP1641; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-SK1 (Ser-225) peptide (SX1645; lanes 2 & 5), or unphosphorylated SK1 (Ser-225) peptide (SX1625; lanes 3 & 6).

Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK1 (Central region) SP1621 (lanes 1 & 2) or anti-SK1 (Ser-225) SP1641 (lanes 3 & 4).

The protein expression levels of DGKα, SphK1 (Cat. SP5421, 1:1000) and p-SphK1(Ser225) (Cat. SP1641) in AML cells after ritanserin treatment for 48 h. Image from publication CC-BY-4.0. PMID: 37392305

In separate experiments, human pulmonary artery ECs were treated with a pharmacologic SphK1 inhibitor, PF-543 (10 µM), 1 h prior to irradiation, followed by Western blotting for p-SphK1 (Cat. SP1641), SphK1, and UCHL1. Representative blots are shown. Image from publication CC-BY-4.0. PMID: 37830619

Western blot image of recombinant his-tagged human SK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-SK1 (Central Region) (SP1621; lanes 1-3) and anti-SK1 (Ser-225) (SP1641; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-SK1 (Ser-225) peptide (SX1645; lanes 2 & 5), or unphosphorylated SK1 (Ser-225) peptide (SX1625; lanes 3 & 6).

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Species Reactivity

Human

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: SP1641

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Sphingosine Kinase 1 (Ser-225)

Scientific Background

Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

SPHK1

Molecular Weight

Immunogen

Phospho-SK1 (Ser-225) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 225 in human SK1. This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and is not homologous to sequences in SK2. The conserved site in rat and mouse SK1 is at serine 224.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to non-specific phosphoserine and unphosphorylated SK1 (Ser-225) peptide before affinity purification using phospho-SK1 (Ser-225) peptide. The purified antibody detects a 47 kDa* full-length recombinant human SK1 protein phosphorylated with ERK2.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-225

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

SK1, SPHK1, SPK1

UniProt ID

Q9NYA1

Shipping

Blue Ice

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Anti-Sphingosine Kinase 1 (Ser-225), Phosphospecific Antibody

SKU: SP1641

Product Details

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