Anti-Slo2.2/Slack K+ Channel Antibody (N3/26)

Name: Anti-Slo2.2/Slack K+ Channel Antibody
Brand: NeuroMab
SKU: 75-051
Price: 394.0 USD
Availability: InStock

Our mouse Slo2.2/Slack K+ channel monoclonal antibody from NeuroMab is produced in-house from clone N3/26. It detects human, marine mollusk, mouse, and rat Slo2.2/Slack K+ channel and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, ELISA.

Immunoblot against brain membranes from adult rat (RBM), Slo2.2 knockout (MBM-Slo2.2 KO) or wild-type (MBM-WT) mice probed with N3/26 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Chris Lingle (Washington University).

Double immunofluorescence staining revealed the expression of the Slack channel (A,D, in red) in somatostatin positive neurons (B,E, in green). Image from publication CC-BY-4.0. PMID:35359569

Immunoblot against brain membranes from adult rat (RBM), mouse (MBM) or human cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] probed with N3/26 (left) or N52A/42 (right) TC supe.

Adult rat hippocampus CA1 immunohistochemistry.

Adult rat medial vestibular nucleus immunohistochemistry.

Adult rat trapezoid body immunohistochemistry.

Double-labeling immunostaining of Slack (red) and TRPV1 (green) in DRGs. Image from publication CC-BY-4.0. PMID: 35626730

Confocal microscopy images obtained from coronal brain slices of c57Bl/6 mice with immunofluorescent labeling of Slack (Cat. 75-051, green, 1:100) and Ank G (red) in neocortex layer 5. Image from publication, CC-BY-4.0. PMID: 38289338.

Immunoblot against brain membranes from adult rat (RBM), Slo2.2 knockout (MBM-Slo2.2 KO) or wild-type (MBM-WT) mice probed with N3/26 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Chris Lingle (Washington University).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-051

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Slo2.2/Slack K+ channel

Scientific Background

Potassium channel subfamily T member 1 or -KCNT1/Slo2.2/Slack K+ channel is encoded by the gene KCNT1. Slo2.2 is a member of the the voltage-gated ion channel (vic) superfamily, and the KCNT protein family, which is made of KCNT1 and KCNT2. Slo2.2 functions as an outwardly rectifying potassium channel subunit to contribute to ion conductance and signaling pathways. Slo2.2 can be activated by high intracellular sodium or chloride levels. Slo2.2 is expressed at the membrane in many areas of the brain, brainstem, spinal cord, spleen and liver. Mutations in this gene cause the early-onset epileptic disorders

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N3/26

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Kcnt1 Slack

Molecular Weight

140 kDa

Immunogen

Fusion protein amino acids 1168-1237 of rat Slo2.2 (accession number NP_068625) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2131855

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:400
ICC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Potassium channel subfamily T member 1 (Sequence like a calcium-activated potassium channel subunit)

UniProt ID

Q9Z258

Shipping

Shipped on ice packs

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Anti-Slo2.2/Slack K+ Channel Antibody (N3/26)

SKU: 75-051

Product Details

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