Anti-Slingshot-1L (Ser-978), Phosphospecific Antibody

Name: Anti-Slingshot-1L (Ser-978), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: SP3901
Price: 348.0 USD
Availability: InStock

Our Slingshot-1L (Ser-978) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Slingshot-1L (Ser-978) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).

Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-SSH1L (C-term.) and anti-SSH1L (Ser-978) antibodies, then detected using appropriate secondary antibody conjugated to Cy3 (Right panel). Phase image of corresponding PC12 cells (Left panel).

DU-145 cells were transfected with control (Ctrl siRNAs) or AKAP2 specific siRNAs (siRNA1 and siRNA2). 72 h after transfection, cell lysates were loaded on SDS-PAGE gels and analyzed by Western blot. Phospho-SSH1 was detected using antibodies recognizing phospho-serines 937 and 978 (Cat. SP3901). The amounts of SSH1 (Cat. SP1711) and AKAP2 were detected using specific antibodies, as indicated. Image from publication CC-BY-4.0. PMID: 37827203

Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).

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Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: SP3901

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Slingshot-1L (Ser-978)

Scientific Background

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell motility. Regulation of cofilin activity can occur through serine phosphorylation and dephosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, Slingshot, PP1, PP2A, PP2B, and chronophin can dephosphorylate Ser-3 and activate actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity. Disruption of SSH1L expression using RNA interference impairs directional cell migration. Phosphorylation of SSH1L at Ser-937 and Ser-978 by PKD leads to association with 14-3-3, sequestration of the phosphatase to the cytoplasm, and reduces cell migration. Thus, Slingshot phosphatases may be critical for regulating cytoskeletal protein activity and cell motility.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

SSH1

Molecular Weight

150

Immunogen

Slingshot-1L (Ser-978) antibody was generated from a phospho-peptide that included amino acids surrounding serine 978 in human SSH1L. This sequence has high homology to the conserved site in rat, mouse, chicken, and zebrafish SSH1L. The sequence also has high homology to the conserved site in SSH2L (Ser-1259).

Immunogen Species

Human

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 150 kDa* full length recombinant human SSH1L phosphoprotein and this reactivity is eliminated with lambda phosphatase treatment. The antibody also detects SSH1L on immunoblots of rat PC12 and mouse C2C12 cells that were stimulated with calyculin A.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-978

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

SSH1L

UniProt ID

Q8WYL5

Shipping

Blue Ice

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Anti-Slingshot-1L (Ser-978), Phosphospecific Antibody

SKU: SP3901

Product Details

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