Anti-pro-Nerve growth factor (proNGF) Antibody

Our Anti-pro-Nerve growth factor (proNGF) sheep polyclonal primary antibody detects human, mouse, and rat pro-Nerve growth factor (proNGF), and is whole serum. It is validated for use in ELISA, IHC-Frozen, Neutralization, WB.

Western blot of proNGF proteins produced in E.Coli. S-080-100 detects the human and mouse form of proNGF as well as the pro-domain peptide. SDS-PAGE: denatured and reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 um); Blocking: 5% skim milk in TBST, 2 hours at RT; Primary antibody: overnight at 4°C (1:1000 dilution); Secondary antibody: anti-sheep-HRP (1/3000), 2 hours at RT; Detection: Chemiluminiscence.
The sheep anti-proNGF antibodies recognize recombinant proNGF, the prodomain of NGF and but not mouse mature NGF (50 ng in each lane).
Western blot of proNGF proteins produced in E.Coli. S-080-100 detects the human and mouse form of proNGF as well as the pro-domain peptide. SDS-PAGE: denatured and reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 um); Blocking: 5% skim milk in TBST, 2 hours at RT; Primary antibody: overnight at 4°C (1:1000 dilution); Secondary antibody: anti-sheep-HRP (1/3000), 2 hours at RT; Detection: Chemiluminiscence.
Western blot of proNGF proteins produced in E.Coli. S-080-100 detects the human and mouse form of proNGF as well as the pro-domain peptide. SDS-PAGE: denatured and reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 um); Blocking: 5% skim milk in TBST, 2 hours at RT; Primary antibody: overnight at 4°C (1:1000 dilution); Secondary antibody: anti-sheep-HRP (1/3000), 2 hours at RT; Detection: Chemiluminiscence.
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Human, Mouse, Rat
ELISA, IHC, Neutralize, WB
Sheep

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Data Sheet

SKU: S-080-100

Volume/Size: 100 µL
Price:
$327.00

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