Anti-Pink1 PTEN Induced Putative Kinase 1 Recombinant Chicken Chimeric mAb (N4/15)

Name: Anti-Pink1 PTEN Induced Putative Kinase 1 Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-060
Price: 470.0 USD
Availability: InStock

Our Anti-Pink1 PTEN induced putative kinase 1 chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N4/15. It detects human, mouse, and rat Pink1 PTEN induced putative kinase 1, and is purified by affinity chromatography. It is great for use in WB,IHC, ICC, ELISA.

Representative section of formalin fixed, paraffin-embedded rat brain stem neurons stained by anti-PTEN Induced Putative Kinase 1 (PINK1) recombinant chicken monoclonal antibody using goat anti-chicken IgY CF®488 secondary antibody (Cat No. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

Western blotting of rat whole brain homogenate with Aves Labs anti-PTEN Induced Putative Kinase 1 (PINK1) recombinant chicken mAb antibody. The chicken mAb was detected with anti-chicken-HRP (Cat No. 80-1000-HRP). Aves Labs Recombinant Chicken anti-PINK1 recognizes endogenous PINK1 protein in rat whole brain homogenate.

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus stained by anti-PTEN Induced Putative Kinase 1 (PINK1) recombinant chicken monoclonal antibody using goat anti-chicken IgY CF®488 secondary antibody (Cat No. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

Representative section of formalin fixed, paraffin-embedded rat brain cortex stained by anti-PTEN Induced Putative Kinase 1 (PINK1) recombinant chicken monoclonal antibody using goat anti-chicken IgY CF®488 secondary antibody (Cat No. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

Representative section of formalin fixed, paraffin-embedded rat brain stem neurons stained by anti-PTEN Induced Putative Kinase 1 (PINK1) recombinant chicken monoclonal antibody using goat anti-chicken IgY CF®488 secondary antibody (Cat No. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: 78-060

Volume: 100 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Pink1 PTEN induced putative kinase 1

Scientific Background

PTEN Induced Kinase 1 is encoded by the gene PINK1. PINK1 is a member of the protein kinase superfamily, Ser/Thr protein kinase family. PINK1 is a serine/threonine protein kinase that protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. PINK1 is highly expressed in the heart, skeletal muscle, and testis. Diseases associated with PINK1 include Parkinson Disease 6, Autosomal Recessive Early-Onset and Parkinson Disease 2, Autosomal Recessive Juvenile.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N4/15

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

PINK1

Molecular Weight

63 kDa

Immunogen

Fusion protein amino acids 112-496 of human Pink1 (accession number NP_115785) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N4/15 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N4/15 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:500-1:1000
IHC: 1:200-1:1000
ICC: 1:200-1:1000

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when tested by Western Blot.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Serine/threonine-protein kinase PINK1, mitochondrial (EC 2.7.11.1) (BRPK) (PTEN-induced putative kinase protein 1)

UniProt ID

Q9BXM7

Shipping

Shipped on ice packs

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Anti-Pink1 PTEN Induced Putative Kinase 1 Recombinant Chicken Chimeric mAb (N4/15)

SKU: 78-060

Product Details

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