Anti-Paxillin (Ser-83), Phosphospecific Antibody

5 Citations

Our Paxillin (Ser-83) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat, mouse Paxillin (Ser-83) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with NGF (200 ng/ml) for 60 min (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Paxillin (PM1071; lane 1 & 2) or rabbit polyclonal anti-Paxillin (Ser-83) phospho-specific antibody (lane 3 & 4).
Immunocytochemical labeling of Ser-83 phosphorylated paxillin in rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal Paxillin (left) and rabbit polyclonal Paxillin (Ser-83, right) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3.
Wesern blot image of cultured mouse osteoblasts +/-TG-interacting factor 1 (Tgif1). Tgif1 deficiency does not alter the abundance or activation of the FA components and Tgif1 does not co-localize with FA complexes. The representive western blot demonstrates the abundance of p-Paxillin (ser83, Cat no PP 1341, 1:1000) and Paxilllin. Actin was used as a loading control. Image from publication, CC-BY-4.0. PMID: 38661167.
Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with NGF (200 ng/ml) for 60 min (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Paxillin (PM1071; lane 1 & 2) or rabbit polyclonal anti-Paxillin (Ser-83) phospho-specific antibody (lane 3 & 4).
Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with NGF (200 ng/ml) for 60 min (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Paxillin (PM1071; lane 1 & 2) or rabbit polyclonal anti-Paxillin (Ser-83) phospho-specific antibody (lane 3 & 4).
Click on image to zoom

Mouse, Rat
ICC, WB
Rabbit

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SKU: PP1341

Volume: 100 µL
Price:
$340.00
Ships: 1-2 business days

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
302545701:500Fan, Y, et al. 2018. Differential Regulation of Adhesion and Phagocytosis of Resting and Activated Microglia by Dopamine. Frontiers in cellular neuroscience, 309.
271813661:200Fusté, N.P., et al. 2016. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nature Communications, 11581.
Immunohistochemistry: Mouse
PMID Dilution Publication
271813661:100Fusté, N.P., et al. 2016. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nature Communications, 11581.
Western Blot: Mouse
PMID Dilution Publication
386611671:500Bolamperti, S., et al. 2024. Tgif1-deficiency impairs cytoskeletal architecture in osteoblasts by activating PAK3 signaling. Elife, RP94265.
341685561:2500Fan, Y, et al. 2021. Salidroside Improves Chronic Stress Induced Depressive Symptoms Through Microglial Activation Suppression. Frontiers in pharmacology, 635762.
302545701:2000Fan, Y, et al. 2018. Differential Regulation of Adhesion and Phagocytosis of Resting and Activated Microglia by Dopamine. Frontiers in cellular neuroscience, 309.
271813661:500Fusté, N.P., et al. 2016. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nature Communications, 11581.
Additional Publications: Unspecified
PMID Publication
25231004Veith, C., et al. 2014. Hypoxia- or PDGF-BB-dependent paxillin tyrosine phosphorylation in pulmonary hypertension is reversed by HIF-1α depletion or imatinib treatment. Thrombosis and Haemostasis, 1288-1303.

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