Our Anti-Neurotrophin-4/5 (NT-4/5) sheep polyclonal primary antibody detects human, mouse, non-human primate, and rat Neurotrophin-4/5 (NT-4/5), and is IgG. It is validated for use in ELISA, IHC-Frozen, Neutralization, WB.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
IgG
Polyclonal
IgG
ELISA, IHC, Neutralize, WB
Sheep
Recombinant human NT4
Human
Human, Monkey, Mouse, Rat
Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Lyophilized
Protein G purified IgG
Lyophilized from a solution containing PBS pH 7.4
WB: 2-10 µg/mL
IHC: 2-10 µg/mL
ELISA: 2-10 µg/mL
Recommended to be used at a concentration of 2-10 µg/mL for inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control.
Unconjugated
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Known to react with NT4 from rat and human, mouse and monkey.
