Anti-Neurofilament medium polypeptide (NF-M) Antibody

Our Anti-Neurofilament medium polypeptide (NF-M) rabbit polyclonal primary antibody detects bovine, chicken, horse, human, mouse, pig, and rat Neurofilament medium polypeptide (NF-M), and is whole serum. It is validated for use in IF, ICC, IHC, WB.

Image of a rat cerebellum section by Immunofluorescence and immunohistochemistry. The section was stained with R-1395-50, Neurofilament medium polypeptide (NF-M), Rabbit pAb, (red, 1:2,000) and co-stained with product M-1650-100, Growth associated protein 43 (GAP43), Mouse mAb, (green, 1:2,000). Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating section was stained. The NF-M antibody strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regions in the molecular layer.
Image of a rat cerebral cortex section by Immunofluorescence and Immunohistochemistry. The section was stained with R-1395-50, Neurofilament medium polypeptide (NF-M), Rabbit pAb, (red) and co-stained with a monoclonal antibody to the beta-adrendergic receptor kinase 1 (green). Product R-1395-50 reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them.
Analysis by western blot of NF-M expression in neuronal tissue lysates with R-1395-50, (green, 1:2,000) with strong bands at 145 kDa (rodent NF-M molecules) and 160 kDa (pig and other larger mammals including humans). Lane 1: protein standard; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord; Lane 6: pig brain; Lane 7: pig spinal cord.
Image of a rat cerebellum section by Immunofluorescence and immunohistochemistry. The section was stained with R-1395-50, Neurofilament medium polypeptide (NF-M), Rabbit pAb, (red, 1:2,000) and co-stained with product M-1650-100, Growth associated protein 43 (GAP43), Mouse mAb, (green, 1:2,000). Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating section was stained. The NF-M antibody strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regions in the molecular layer.
Image of a rat cerebellum section by Immunofluorescence and immunohistochemistry. The section was stained with R-1395-50, Neurofilament medium polypeptide (NF-M), Rabbit pAb, (red, 1:2,000) and co-stained with product M-1650-100, Growth associated protein 43 (GAP43), Mouse mAb, (green, 1:2,000). Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating section was stained. The NF-M antibody strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regions in the molecular layer.
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Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
ICC, IF, IHC, WB
Rabbit

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Data Sheet

SKU: R-1395-50

Volume/Size: 50 µL
Price:
$327.00

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