Anti-Neu-N (Fox 3) Antibody

Name: Anti-Neu-N (Fox 3) Antibody
Brand: Aves Labs
SKU: NeuN-1010
Price: 295.0 USD
Availability: InStock

Our Anti-Neu-N primary antibody from Aves Labs is chicken polyclonal. It detects human, mouse, and rat Neu-N and is total IgY fraction. It is great for use in WB, IHC, ICC.

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing cells stained by anti-NeuN (Rbfox3) antibody. The sections were stained with Aves Labs anti-NeuN polyclonal antibody (visualized in green, Cat No. NeuN-1010). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing cells stained by anti-NeuN (Rbfox3) antibody. The sections were stained with Aves Labs anti-NeuN polyclonal antibody (visualized in green, Cat No. NeuN-1010). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing cells stained by anti-NeuN (Rbfox3) antibody. The sections were stained with Aves Labs anti-NeuN polyclonal antibody (visualized in green, Cat No. NeuN-1010). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: NeuN-1010

Volume: 100 µL

Variant

Price:

$295.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Neu-N (Fox 3)

Scientific Background

Human Neu-N (Fox 3) is a 33,873 dalton (312 amino acid) RNA-binding protein. This protein is found in the nuclei of virtually all post-mitotic central and peripheral neurons and has been used extensively as a general neuronal marker. In these studies, Neu-N (Fox 3) stains neuronal nuclei relatively uniformly, except in the region of the nucleolus, providing a distinctive donut (i.e., torus) shape. Since this antigen is not seen in the cytoplasm, it offers the advantage of being useful in co-immunostaining studies with rabbit and mouse antibodies against various cytoplasmic neuronal antigens.

Format

Total IgY fraction

Concentration

Lot dependent

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

RBFOX3

Molecular Weight

46, 48 kDa

Immunogen

N-terminal 100 amino acids of human FOX3 expressed in and purified from E. coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Total IgY fraction

Buffer

Concentrated IgY preparation plus 0.02% NaN3

Dilution Ranges

WB: 1:250-1:500
WB Brain: 1:250-1:500
IHC: 1:2000 - 1:4000
ICC: 1:2000 - 1:4000

Conjugate

Unconjugated

Specificity

Does not cross react with Fox1 or Fox2

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, NeuN antigen)

UniProt ID

A6NFN3

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Anti-Neu-N (Fox 3) Antibody

SKU: NeuN-1010

Product Details

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