Our Anti-Munc18-1 (Ser241) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Munc18-1 (Ser241) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~65 kDa Munc18-1 protein phosphorylated at Ser241 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc18-1 is thought to be regulated by phosphorylation.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, WB
Rabbit
STXBP1
65 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser241 of mouse Munc18-1, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:500
Unconjugated
Specific for endogenous levels of the ~65 kDa Munc18-1 protein phosphorylated at Ser241. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser241
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
