Anti-Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) Antibody
Our Anti-Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) rabbit polyclonal primary antibody detects human and rat Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A), and is whole serum. It is validated for use in ICC, WB.








Confocal microscopy on immunofluorescently detected MAP1LC3 A in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A : whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A appearing in red and MHC class II appearing in. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
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SKU: R-146-100
Product Details
Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A)
MAP1A and MAP1B are microtubule-associated protein which mediate the physical interactions between microtubules and components of the cytoskeleton (probably involved in autophagosome formation). MAP1A and MAP1B each consist of a heavy chain subunit and 3 different light chain subunits (LC1, LC2 and LC3). MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor form of MAP1LC3A is cleaved by APG4/ATG4B to form the cytosolic form LC3-1. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. MAP1LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but is absent in thymus and peripheral leukocytes.
Whole serum
Polyclonal
Mixed
ICC, WB
Rabbit
A synthetic peptide (RSFADRCKEVQQI) corresponding to the N-terminal of human MAP1LC3 A protein conjugated to Blue Carrier Protein has been used as the immunogen. The sequence is homologous with mouse and rat MAP1LC3 A.
Human
Human, Rat
Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Lyophilized
WB: 1:500
IHC: 1:100-1:1000
ICC: 1:100-1:1000
IHC: 1:100-1:1000
ICC: 1:100-1:1000
IHC: 4% PFA fixed or 2% PLP fixation, ICC: acetone fixed specimens recommended. WB: R-146-100 requires samples to be denatured ONLY by boiling in SDS solution, not reduced. No signal is achieved using reduced samples. (Samples treated with DTT or Beta mercaptoethanol). Overnight 4 degrees is recommended for primary antibody dilution in WB.
Unconjugated
IHC, WB and ELISA confirmed the specificity for MAP1LC3 A. Human, rat. Other species not yet tested.
For research use only.
United States
12 months after date of receipt (unopened vial).
Microtubule-associated proteins 1A/1B light chain 3A; MAP1A/MAP1B LC3 A; MAP1A/1B light chain 3 A; MAP1 light chain 3-like protein 1; Microtubule-associated protein 1 light chain 3 alpha; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; APG8a; MAP1LC3A
25°C (ambient)