Anti-MAP1LC3C Antibody
Our Anti-Microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C) rabbit polyclonal primary antibody detects human Microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C), and is whole serum. It is validated for use in ICC, IHC-Frozen, WB.





Confocal microscopy on immunofluorescently detected MAP1LC3 C in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 C : whole serum (R-140-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The detected MAP1LC3 C appears red. The cells were also stained for Myeloperoxidase (MPO) appearing in green. The cells were counter stained with Hoechst Dye(blue colour). Here, the merged picture is presented.
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SKU: R-140-100
Product Details
Microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C)
FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: LC3-I: Cytoplasm. LC3-II: Intracytoplasmic membrane; lipid-anchor. LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in placenta, lung and ovary. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. SIMILARITY: Belongs to the MAP1 LC3 family.
Whole serum
Polyclonal
Mixed
ICC, IHC, WB
Rabbit
A synthetic peptide (CQEEVAGIRAKF) corresponding to the N-terminal of human MAP1LC3 C conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence deriven from MAP1LC3 C protein in Macaca mulatta (monkey) and Canis familiaris (dog).
Human
Human
Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Lyophilized
WB: 1:100-1:1000
IHC: 1:100-1:1000
IHC: 1:100-1:1000
Unconjugated
IHC and WB confirmed the specificity for MAP1LC3 C. This antibody should recognize MAP1LC3 C only and not the other forms MAP1LC3A and MAP1LC3B. Human, other species have not yet been tested.
For research use only.
United States
12 months after date of receipt (unopened vial).
Microtubule-associated proteins 1A/1B light chain 3C; Microtubule-associated protein 1 light chain 3 gamma; MAP1A/MAP1B LC3 C; MAP1A/1B light chain 3 C; MAP1 light chain 3-like protein 3; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; APG8c; MAP1LC3C
25°C (ambient)