Anti-Kv2.2 Potassium Channel Antibody (N372B/1)

Name: Anti-Kv2.2 Potassium Channel Antibody
Brand: NeuroMab
SKU: 75-369
Price: 337.0 USD
Availability: InStock

Our mouse Kv2.2 potassium channel monoclonal antibody from NeuroMab is produced in-house from clone N372B/1. It detects ferret, human, mouse, non-human primate, and rat Kv2.2 potassium channel and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, ELISA.

Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/1 (red) and Hoechst nuclear stain (blue).

Adult rat whole brain immunohistochemistry.

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged Kv2.2long, Myc-tagged Kv2.2short or untagged Kv2.1 plasmid; and adult brain membranes from rat (RBM), Kv2.2 wild-type (WT) and knockout (KO) mice probed with N372B/1 (left) or N52A/42 (right) TC supe.

Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/1 (red) and Hoechst nuclear stain (blue).

Click on image to zoom

Species Reactivity

Ferret, Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-369

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Kv2.2 potassium channel

Scientific Background

Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N372B/1

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Kcnb2

Molecular Weight

120 kDa

Immunogen

Fusion protein amino acids 717-907 (cytoplasmic C-terminus) of rat Kv2.2 long isoform (accession number Q63099) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Ferret, Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2315870

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Kv2.2 short isoformDoes not cross-react with Kv2.1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Potassium voltage-gated channel subfamily B member 2 (CDRK) (Voltage-gated potassium channel subunit Kv2.2)

UniProt ID

Q63099

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-Kv2.2 Potassium Channel Antibody (N372B/1)

SKU: 75-369

Product Details

Bulk Order Anti-Kv2.2 Potassium Channel Antibody

Related Products

Recently Viewed