Anti-Internexin alpha (Alpha-Inx) Antibody (1D2)

Name: Anti-Internexin alpha (Alpha-Inx) Antibody
Brand: Biosensis
SKU: M-1378-250
Price: 327.0 USD
Availability: InStock

Our Anti-Internexin alpha (Alpha-Inx) mouse monoclonal primary antibody detects cat, human, mouse, other mammals, and rat Internexin alpha (Alpha-Inx), and is IgG. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.

Mixed cultures of rat CNS cells stained with Mouse monoclonal antibody to Internexin alpha [1D2] M-1378-250 (red) and Chicken polyclonal antibody to Microtubule associated protein 2 C-1382-50 (green). The internexin alpha is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.

Left: Analysis of alpha-internexin expression in rat cerebellum with mouse antibody to alpha-internexin (1:5,000, green) by Immunohistochemistry. Co-staining was performed with chicken antibody to calretinin (C-1801-50, 1:2,000, red). IHC Method: Following transcardial perfusion of rat with 4% PFA, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The alpha-internexin antibody selectively stains neuronal processes, in particular parallel fibers, the axons of granule cells. Calretinin antibody stains interneurons predominantly in the molecular layer of the cerebellum. Right: Western blot analysis of alpha-internexin expression in tissue lysates using mouse antibody to alpha-internexin (1:10,000, green): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord, [7] cow spinal cord. Alpha-internexin protein migrates on the gel with apparent molecular weight of 64 to 66 kDa with slight variability among species.

Mixed cultures of rat CNS cells stained with Mouse monoclonal antibody to Internexin alpha [1D2] M-1378-250 (red) and Chicken polyclonal antibody to Microtubule associated protein 2 C-1382-50 (green). The internexin alpha is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.

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Species Reactivity

Feline, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1378-250

Volume/Size: 250 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Internexin alpha (Alpha-Inx)

Scientific Background

Neurofilaments can be defined as the intermediate or 10 nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.

Format

IgG

Clonality

Monoclonal

Clone

1D2

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Molecular Weight

64-66 kDa

Immunogen

Recombinant rat alpha-internexin expressed and purified from E. coli

Immunogen Species

Rat

Species Reactivity

Feline, Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute with 250 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:1000-1:5000
ICC: 1:250-1:500

Application Details

The epitope recognized by this antibody is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation so this antibody is ideally suited for studies of paraffin embedded formalin fixed histological sections.

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Hu, rat, Ms, Fel, and other mammals.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

66 kDa neurofilament protein; NF-66; Neurofilament-66; Alpha-internexin; Alpha-Inx; Neurofilament 5; INA; NEF5;

UniProt ID

P23565

Shipping

25°C (ambient)

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Anti-Internexin alpha (Alpha-Inx) Antibody (1D2)

SKU: M-1378-250

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