Anti-IκBα (Tyr-42), Phosphospecific Antibody

Name: Anti-IκBα (Tyr-42), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: IP1031
Price: 340.0 USD
Availability: InStock

8 Citations

Our IκBα (Tyr-42) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat IκBα (Tyr-42) and is antigen affinity purified. It is great for use in WB, IP.

Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IκBα (lane 1), anti-IκBα (Tyr-42) (IP1031; lanes 2-5), or anti-IκBα (Tyr-305) (IP1041; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IκBα (Tyr-42) (lane 3 & 8) or IκBα (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).

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Species Reactivity

Human, Mouse, Rat

Applications

IP, WB

Host Species

Rabbit

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SKU: IP1031

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

IκBα (Tyr-42)

Scientific Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

IP, WB

Host Species

Rabbit

Gene Name

NFKBIA

Molecular Weight

Immunogen

IκBα (Tyr-42) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 42 of human IκBα. This peptide sequence has low homology to other IκB proteins, but does have some homology to unrelated proteins that may have a conserved tyrosine phosphorylation motif.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IP: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IκBα (Tyr-42) peptide (without carrier). The antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of A431 and Jurkat cells treated with pervanadate, but not in control cells. Due to homologies to tyrosine sites on other proteins, it is recommended that the antibody be used to detect phosphorylation of immunoprecipitated IκBα.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-42

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

IkB, MAD3, IkappaBalpha, NFkappaB inhibitor IkBa

UniProt ID

P25963

Shipping

Blue Ice

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat

Western Blot: Human
PMID	Dilution	Publication
28921811	not listed	Zhao, X, et al. 2018. A role for the non-receptor tyrosine kinase ACK1 in TNF-alpha-mediated apoptosis and proliferation in human intestinal epithelial caco-2 cells. Cell biology international, 1097-1105.
25671697	not listed	Cullen, S., et al. 2015. Redox-regulated pathway of tyrosine phosphorylation underlies NF-κB induction by an atypical pathway independent of the 26S proteasome. Biomolecules, 95-112.
17533369	not listed	Sethi, G., et al. 2007. Epidermal growth factor (EGF) activates nuclear factor-kappaB through IkappaBalpha kinase-independent but EGF receptor-kinase dependent tyrosine 42 phosphorylation of IkappaBalpha. Oncogene, 7324-7332.

Western Blot: Mouse
PMID	Dilution	Publication
29175419	not listed	Zhang, Y, et al. 2018. Exogenous oxidants activate nuclear factor kappa B through Toll-like receptor 4 stimulation to maintain inflammatory phenotype in macrophage. Biochemical pharmacology, 104-118.
19835847	not listed	Cullen, S.J., et al. 2010. Proteasome inhibition up-regulates inflammatory gene transcription induced by an atypical pathway of NF-kappaB activation. Biochemical pharmacology, 706-714.
18768868	1:1000	Naidu, S., et al. 2008. An atypical NF-kappa B-regulated pathway mediates phorbol ester-dependent heme oxygenase-1 gene activation in monocytes. Journal of Immunology, 4113-4123.
17426256	not listed	Boosani, C.S., et al. 2007. Regulation of COX-2 mediated signaling by alpha3 type IV noncollagenous domain in tumor angiogenesis. Blood, 1168-1177.

Western Blot: Rat
PMID	Dilution	Publication
19074556	not listed	Wright, C.J., et al. 2009. Hyperoxia-induced NF-kappaB activation occurs via a maturationally sensitive atypical pathway. American Journal of Physiology. Lung Cellular and Molecular Physiology, L296-L306.

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Anti-IκBα (Tyr-42), Phosphospecific Antibody

SKU: IP1031

Product Details

Product Specific References for Applications and Species

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