Anti-Histone H2B (C-terminus) Antibody

Name: Anti-Histone H2B (C-terminus) Antibody
Brand: PhosphoSolutions
SKU: HP4291
Price: 340.0 USD
Availability: InStock

2 Citations

Our Histone H2B (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, rat, and Xenopus Histone H2B (C-terminus) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B (C-terminus) (lanes 1 & 2), anti-Histone H2B (Ser-36) (lanes 3 & 4), and anti-Histone H2B (a.a. 33-47) (lanes 5 & 6).

Immunocytochemical labeling of Histone H2B in methanol and acetone fixed rat A7r5 cells. The cells were labeled with rabbit polyclonal Histone H2B (C-terminus) antibody (HP4291), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B (C-terminus) (lanes 1 & 2), anti-Histone H2B (Ser-36) (lanes 3 & 4), and anti-Histone H2B (a.a. 33-47) (lanes 5 & 6).

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Species Reactivity

Frog, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: HP4291

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Histone H2B (C-terminus)

Scientific Background

The nucleosome is a protein complex consisting of four core histones (H2A, H2B, H3, and H4). Two molecules of each histone forms an octamer that makes up the nucleosome. DNA wraps around repeating nucleosome units to generate chromatin structures. The structure of chromatin determines the accessiblity to transcription factors. Post-translational modification of the amino-terminal tail of histones in nucleosomes alters chromatin structure to promote or inhibit transcription. Complex alterations in acetylation, methylation, ubiquination, and/or phosphorylation determine the chromatin structural changes that occur during specific phases of the cell cycle or in response to cell stimuli. One mode of regulating histone H2B activity is through phosphorylation in the amino terminal region. Important sites of phosphorylation include Ser-14, Ser-32, and Ser-36. AMPK phosphorylates Ser-36 on histone H2B during cell stress leading to increased transcription and cell survival, while ectopic expression of an unphosphorylatable histone H2B during cell stress reduces transcription of AMPK-dependent genes and lowers cell survival.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

H2BC3

Molecular Weight

Immunogen

Histone H2B synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminus of human histone H2B. This sequence is well conserved in rat, mouse, fish, and drosophila histone H2B, and has low homology to other histone family members.

Immunogen Species

Human

Species Reactivity

Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:5000
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects a 15 kDa* protein corresponding to the molecular mass of Histone H2B on SDS-PAGE immunoblots of human A431, Jurkat, HeLa, mouse C2C12, rat PC12, and A7r5 cells. The antibody also shows nuclear labeling in immunocytochemistry of methanol/acetone fixed cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

HIST1H2BB, H2BFF, Histone H2B type1B, H2B/f

UniProt ID

P33778

Shipping

Blue Ice

Product Specific References for Applications and Species

Western Blot: Human | Insect

Western Blot: Human
PMID	Dilution	Publication
24137500	not listed	Seo, Y.J., et al. 2013. Sphingosine kinase 1 serves as a pro-viral factor by regulating viral RNA synthesis and nuclear export of viral ribonucleoprotein complex upon influenza virus infection. PLoS One, e75005.

Western Blot: Insect
PMID	Dilution	Publication
24036077	not listed	Soe, K.C., et al. 2013. Transcriptional coactivator CIITA, a functional homolog of TAF1, has kinase activity. Biochimica et Biophysica Acta, 11844-1190.

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Anti-Histone H2B (C-terminus) Antibody

SKU: HP4291

Product Details

Product Specific References for Applications and Species

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