Anti-Histidine-tag (His) Antibody (HIS.H8)

Name: Anti-Histidine-tag (His) Antibody
Brand: Biosensis
SKU: M-1311-100
Price: 377.0 USD
Availability: InStock

Our Anti-Histidine-tag (His) mouse monoclonal primary antibody detects Histidine-tag (His), and is IgG. It is validated for use in ICC, WB.

Applications

ICC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1311-100

Volume/Size: 100 µg

Variant

Price:

$377.00

Quantity:

Product Details

Target

Histidine-tag (His)

Scientific Background

The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.

Format

IgG

Clonality

Monoclonal

Clone

HIS.H8

Isotype

IgG2b

Applications

ICC, WB

Host Species

Mouse

Immunogen

Synthetic peptide containing 6 Histidine residues.

Immunogen Species

Species Independent

Storage

Stable for 1 year at -20°C unopened. After opening we recommend aliquoting and storing at -20°C for up to 6 months. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap.

Physical State

Liquid

Buffer

PBS, pH 7.4 with 0.05% sodium azide

Dilution Ranges

WB: 1:1000
IHC: 1:200
ICC: 1:200
IP: 1:200

Conjugate

Unconjugated

Specificity

His-tagged fusion proteins.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Shipping

2-8°C (on cold packs)

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Anti-Histidine-tag (His) Antibody (HIS.H8)

SKU: M-1311-100

Product Details

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