Anti-Glutathione peroxidase 1 (GPx-1) Antibody

Name: Anti-Glutathione peroxidase 1 (GPx-1) Antibody
Brand: Biosensis
SKU: S-072-100
Price: 337.0 USD
Availability: InStock

Our Anti-Glutathione peroxidase 1 (GPx-1) sheep polyclonal primary antibody detects human and rat Glutathione peroxidase 1 (GPx-1), and is whole serum. It is validated for use in ELISA, IHC-Frozen, IHC-Paraffin-embedded, WB.

Immunofluorescence histochemistry with sheep antibody to glutathione peroxidase 1 (S-072-100). Staining obtained within microglia in the septal region of rat brain and is doubled labeled to show the presence of GFP protein in these same cells following a transfection experiment. Concentration of antibody used was 1:500.

Anti-Glutathione peroxidase 1 (GPx-1) Antibody

Immunofluorescence histochemistry with sheep antibody to glutathione peroxidase 1 (S-072-100). Staining obtained within microglia in the septal region of rat brain and is doubled labeled to show the presence of GFP protein in these same cells following a transfection experiment. Concentration of antibody used was 1:500.

Click on image to zoom

Species Reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host Species

Sheep

Go to Full Product Details

Data Sheet

SKU: S-072-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Product Details

Target

Glutathione peroxidase 1 (GPx-1)

Scientific Background

Glutathione peroxidase 1 has a role in detoxification of hydrogen peroxide and is one of the most important antioxidant enzymes in humans. It exists as a homotetramer which localises to the cytoplasm. It belongs to the glutathione peroxidase family. Glutathione peroxidase 1 is one of few proteins in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. This protein has a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine repeats. The allele with five alanine repeats is significantly associated with breast cancer risk. Two alternatively spliced isoforms have been identified.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ELISA, IHC, WB

Host Species

Sheep

Molecular Weight

22 kDa

Immunogen

A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human lutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.

Immunogen Species

Human

Species Reactivity

Human, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.

Physical State

Lyophilized

Dilution Ranges

WB: 1:10000-1:100000
IHC: 1:2000-1:20000
ELISA: 1:2000-1:20000

Application Details

Antigen retrieval has been used in testing but may not be necessary. This antiserum has extremely high titer, it stains human and rat brain microglia intensely and to some extent also stains neurons. Other tissues have not yet been tested. On Western blotting under reducing conditions recognizes a 22 kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes.

Conjugate

Unconjugated

Specificity

This antibody has been shown to be specific for glutathione peroxidase 1 (GPx-1) protein. Human and rat, other species have not yet been tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

GSHPx-1; GPx-1; Cellular glutathione peroxidase; GPX1

UniProt ID

P07203

Shipping

25°C (ambient)

Recover password

Create my account

Anti-Glutathione peroxidase 1 (GPx-1) Antibody

SKU: S-072-100

Product Details

Bulk Order Anti-Glutathione peroxidase 1 (GPx-1) Antibody

Related Products

Recently Viewed