Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

Our Anti-Glial Fibrillary Acidic Protein (GFAP) chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Glial Fibrillary Acidic Protein (GFAP), and is IgY fraction. Works in IHC, ICC, WB.

Glial Fibrillary Acidic Protein (GFAP) immunostaining (red) of mixed cortical mouse brain cultures (1:500 dilution). Secondary antibody is Texas Red goat anti-chicken IgY. The blue is DAPI staining of nuclei of all cells, including non-GFAP-positive cells. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.
Representative section of formalin fixed, paraffin-embedded rat brain showing staining of GFAP. The sections were stained with Aves Labs anti-GFAP antibody (visualized in green) (Cat. GFAP) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.
Immunohistochemical staining of GFAP-positive radial glial cells in the periventricular zone of an e16 mouse brain. Section was a vibratome, thick section (20 um) using a lightly-fixed (2% paraformaldehyde) mouse brain. 1˚ antibody (GFAP) was used at 1:500 dilution; 2˚ antibody (HRP-goat anti-chicken IgY) was used at 1:500 dilution.
Chicken anti-Myelin Basic Protein (MBP) (green) and Mouse anti-GFAP (red) with nuclei stained with Hoechst dye (blue).
Chicken anti-NSE (green) and mouse anti-GFAP (red).
Immunostaining of rat hippocampus CA1 region identifying NeuN (red), IBA1 (green), and GFAP (Cat. GFAP, 1:4000, cyan). The 30 um free-floating PFA-fixed tissue was embedded with Prolong Diamond with DAPI (grey). 10x image. Image kindly provided by Tim D. Ostrowski, A.T. Still Univ., Kirksville, MO.
Immunofluorescent staining of WT or Ncf1–/– mouse neuronal stem cells (NSCs) for Olig 4 (O4), ßIII Tubulin (Tuj1) and Gfap (Cat. GFAP, 1:250). Image from publication CC-BY-4.0. PMID: 36707536
High manification view of immunofluorescent staining of WT or Ncf1–/– mouse neuronal stem cells (NSCs) for Olig 4 (O4), ßIII Tubulin (Tuj1) and Gfap (Cat. GFAP, 1:250). Image from publication CC-BY-4.0. PMID: 36707536
Immunohistochemistry (Formalin-fixed paraffin-embedded) staining of adult mouse brainstem showing labeling of astrocytes with chicken anti-GFAP antibody (Cat. GFAP) at a dilution of 1:500.
GFAP (pink, 1:500, Cat no GFAP) and DAPI (blue) staining were conducted on C57BL/6J mice at P35, two days after inducing a focal stroke in the motor cortex. The image depicts local upregulation of GFAP+ astrocytes in the motor cortex. Image kindly provided by Moawiah Naffa'A, Ph.D., Duke University, Neurobiology.
Immunostaining of astrocytes isolated from mouse cortex showing specific detection of GFAP (Cat. GFAP, 1:500, red). Nuclei are labeled with DAPI (blue). Image kindly provided by Haofei Ni, Icahn School of Medicine at Mount Sinai.
Immunostaining of astrocytes isolated from mouse cortex showing specific detection of GFAP (Cat. GFAP, 1:500, red). Nuclei are labeled with DAPI (blue). Image kindly provided by Haofei Ni, Icahn School of Medicine at Mount Sinai.
Representative confocal z stack images and bar graphs showing MERTK (green) expression in control and hCOs astrocytes (GFAP) (Cat. GFAP, 1:1000; red). Image from publication CC-BY-4.0. PMID:37527657
Immunostaining of adult mouse brain section fixed in 4% paraformaldehyde and preserved in cryoprotectant showing specific detection of GFAP (Cat. GFAP, 1:500; red) in astrocytes around the corpus calosum. Nuclei stained with DAPI (blue). Background stain was attenuated with TrueVIEW. Image kindly provided by Ifeoluwa (Hiphy) Awogbindin of the Tremblay Lab, Division of Medical Sciences, University of Victoria, Canada.
Glial Fibrillary Acidic Protein (GFAP) immunostaining (red) of mixed cortical mouse brain cultures (1:500 dilution). Secondary antibody is Texas Red goat anti-chicken IgY. The blue is DAPI staining of nuclei of all cells, including non-GFAP-positive cells. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.
Glial Fibrillary Acidic Protein (GFAP) immunostaining (red) of mixed cortical mouse brain cultures (1:500 dilution). Secondary antibody is Texas Red goat anti-chicken IgY. The blue is DAPI staining of nuclei of all cells, including non-GFAP-positive cells. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.
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Human, Mouse, Non-Human Primate, Rat
Flow, ICC, IHC, WB
Chicken

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Data Sheet

SKU: GFAP

Volume: 200 µL
Price:
$301.00
Ships: 1-2 business days

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