Anti-GIT1 Antibody (N39B/8)

Name: Anti-GIT1 Antibody
Brand: NeuroMab
SKU: 75-094
Price: 337.0 USD
Availability: InStock

Our mouse GIT1 monoclonal antibody from NeuroMab is produced in-house from clone N39B/8. It detects human, mouse, and rat GIT1 and is purified by Protein A chromatography. KO validated. Works in WB, IHC, IP, ELISA.

Sliced human breast tumor was stained using Anti-GIT1 antibody (NeuroMab, Cat. No. 75-094, Clone N39/8, 1000x), then followed by anti-Mouse IgG (H+L) secondary antibody, Alexa FluorTM (Invitrogen, A-21425) and DAPI for nuclei. Scale bar 10 μm. Image kindly provided by Songbai Zhang, Karolinska Institutet, Stockholm, Sweden.

Adult rat hippocampus immunohistochemistry.

Immunoblot versus lysates of HEK293T cells untransfected and transfected with Flag-tagged GIT1 and GIT2 plasmids and probed with N39B/8 (top) and anti-Flag (bottom) antibodies. Data courtesy of Eun-Kyoung Hahm, Jaewon Ko, and Eunjoon Kim, Korea Advanced Institute of Science and Technology.

Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].

Sliced human breast tumor was stained using Anti-GIT1 antibody (NeuroMab, Cat. No. 75-094, Clone N39/8, 1000x), then followed by anti-Mouse IgG (H+L) secondary antibody, Alexa FluorTM (Invitrogen, A-21425) and DAPI for nuclei. Scale bar 10 μm. Image kindly provided by Songbai Zhang, Karolinska Institutet, Stockholm, Sweden.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-094

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GIT1

Scientific Background

G-protein coupled receptor kinase interacting ArfGAP 1 is encoded by the gene GIT1. GIT1 is a member of the GIT protein family. GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. GIT1 is expressed in numerous tissues with the highest levels in the testis. Diseases associated with GIT1 include Attention Deficit-Hyperactivity Disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N39B/8

Isotype

IgG1

Applications

ELISA, IHC, IP, WB

Host Species

Mouse

Gene Name

Git1

Molecular Weight

90 kDa

Immunogen

Fusion protein amino acids 375-770 (C-terminus) of rat GIT1(accession number Q9Z272) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2109991

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.21

Conjugate

Unconjugated

Specificity

No cross-reactivity against GIT2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1)

UniProt ID

Q9Z272

Shipping

Shipped on ice packs

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Anti-GIT1 Antibody (N39B/8)

SKU: 75-094

Product Details

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