Anti-GFP Recombinant Chicken Chimeric mAb Biotin Conjugate (N86/38)

Name: Anti-GFP Recombinant Chicken Chimeric mAb Biotin Conjugate
Brand: Aves Labs
SKU: 78-132-BTN
Price: 562.0 USD
Availability: InStock

Our chicken GFP biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone N86/38. It detects n/a GFP and is purified by affinity chromatography. Works in ELISA, IHC, ICC, WB, IP.

Anti-GFP Recombinant Chicken Chimeric mAb Biotin Conjugate

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Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-132-BTN

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

GFP

Scientific Background

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

N86/38

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

GFP

Molecular Weight

27 kDa

Immunogen

Fusion Protein Amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Immunogen Species

Jellyfish

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

IHC: 1:500-1:1000
ICC: 1:500-1:1000

Conjugate

Biotin

Specificity

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Does not cross-react with Red fluorescent protein (dsRed)

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Green fluorescent protein

UniProt ID

P42212
UniProt Summary: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

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Anti-GFP Recombinant Chicken Chimeric mAb Biotin Conjugate (N86/38)

SKU: 78-132-BTN

Product Details

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