Anti-GABA-A-R, Beta3 Antibody (N87/25)

Name: Anti-GABA-A-R, Beta3 Antibody
Brand: NeuroMab
SKU: 75-149
Price: 337.0 USD
Availability: InStock

Our mouse GABA(A)R, Beta3 monoclonal antibody from NeuroMab is produced in-house from clone N87/25. It detects human, mouse, and rat GABA(A)R, Beta3 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Immunofluorescence of cultured hippocampal neurons with N87/25 (red) and GABA-A-R-Gamma2 rabbit polyclonal (green). Images courtesy of Angel de Blas (University of Connecticut).

Adult rat hippocampus immunohistochemistry.

Whole adult rat brain immunohistochemistry.

$Immunoblot against membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N87/25 (left) or N52A/42 (right) TC supe.$

Western blot image of human BT549 cells labeling GABA-A, Beta 3 (Cat. 75-149, 1:1000). The cells were transduced with different lentiviral mediated GABAA beta 3 subunit knockdown constructs (lane 2-5). The image was kindly provided by Asha Suryanaraayanan, Department of Pharmaceutical Sciences, Saint Joseph's University.

Adult rat brain membrane immunoblot.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-149

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GABA(A)R, Beta3

Scientific Background

Gamma-aminobutyric acid receptor subunit beta-3 or GABA AR-Beta3 is encoded by the gene GABRB3. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR-beta3 a is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR beta3 has been found to be expressed in retina, heart and primarily in brain at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. This gene may be associated with the pathogenesis of several disorders including epilepsy and autism, childhood absence epilepsy 5 and early infantile epileptic encephalopathy 43.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N87/25

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Gabrb3 Gabrb-3

Molecular Weight

55 kDa

Immunogen

Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta3 (accession number AAB60502) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2109585

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.76

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500
IP: 2 ug

Conjugate

Unconjugated

Specificity

No cross-reactivity against GABA-A-R-Beta1 or –Beta2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Gamma-aminobutyric acid receptor subunit beta-3 (GABA(A) receptor subunit beta-3)

UniProt ID

P63080

Shipping

Shipped on ice packs

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Anti-GABA-A-R, Beta3 Antibody (N87/25)

SKU: 75-149

Product Details

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