Anti-Endomorphin-1 and Endomorphin-2 (E-1/E-2) Antibody

Name: Anti-Endomorphin-1 and Endomorphin-2 (E-1/E-2) Antibody
Brand: Biosensis
SKU: R-1495-50
Price: 337.0 USD
Availability: InStock

Our Anti-Endomorphin-1 and Endomorphin-2 (E-1/E-2) rabbit polyclonal primary antibody detects human, mouse, and rat Endomorphin-1 and Endomorphin-2 (E-1/E-2), and is affinity-purified. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded.

Immunohistochemical staining in rat ventral periaqueductal grey matter (PAG). 4% paraformaldehyde fixed rat brain crystostat sections (10 µm) were incubated overnight at 4°C with Rabbit polyclonal antibody to Endomorphin-1 and Endomorphin-2 (10 µg/mL) followed by incubation with donkey anti-rabbit Rhodamine Red conjugated secondary antibody (1:200).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Rabbit

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Data Sheet

SKU: R-1495-50

Volume/Size: 50 µg

Variant

Price:

$337.00

Quantity:

Product Details

Target

Endomorphin-1 and Endomorphin-2 (E-1/E-2)

Scientific Background

Endomorphins 1 and 2 are endogenous opioid peptides which have the highest affinity for the mu-opioid receptors. Located in various parts of the brain and interacts with mu-opioid receptors and produces analgesia.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC

Host Species

Rabbit

Immunogen

Antibodies to human Endomorphins 1 and 2 were made by immunising rabbits with the human Endomorphin-1 peptide (YPWF) and then selecting those antibodies that cross-reacted with both human Endomorphins 1 and 2.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute in 0.05 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material. At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles

Physical State

Lyophilized

Buffer

Lyophilized with BSA.

Dilution Ranges

IHC: 5-15 µg/mL
ICC: 5-15 µg/mL

Conjugate

Unconjugated

Specificity

Human; mouse; rat. Endomorphin-1 is highly conserved molecule so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-2 = 70%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

EM 1; EM 2;

Shipping

25°C (ambient)

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Anti-Endomorphin-1 and Endomorphin-2 (E-1/E-2) Antibody

SKU: R-1495-50

Product Details

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