Anti-eEF2K (Ser-500), Phosphospecific Antibody

Name: Anti-eEF2K (Ser-500), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: EP4451
Price: 340.0 USD
Availability: InStock

6 Citations

Our eEF2K (Ser-500) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat eEF2K (Ser-500) and is antigen affinity purified. It is great for use in WB.

Recombinant human eEF2K untreated (lanes 1 & 4), auto-phosphorylated in the presence of Ca2+ and Calmodulin (lanes 2 & 5), and dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-500) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.)

Western blot image of C2C12 untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-eEF2K (N-terminus) (lanes 1 & 2) and anti-eEF2K (Ser-500) (lanes 3 & 4).

Recombinant human eEF2K untreated (lanes 1 & 4), auto-phosphorylated in the presence of Ca2+ and Calmodulin (lanes 2 & 5), and dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-500) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.)

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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Data Sheet

SKU: EP4451

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

eEF2K (Ser-500)

Scientific Background

Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. eEF2 kinase (eEF2K) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation. eEF2K is normally dependent on Ca2+ ions and calmodulin, and can be activated by PKA in response to elevated cAMP levels during cell stress- or starvation-related conditions. Regulation of eEF2K occurs through phosphorylation at multiple sites. Ser-78 phosphorylation is required for calmodulin binding and eEF2K activity, while phosphorylation of Ser-500 is required for Ca2+/calmodulin-independent kinase activity. Thr-348 is an autophosphorylation site that is required for kinase activity. Inhibitory phosphorylation may also regulate eEF2K, since phosphorylation at Ser-359 by SAPK4/p38δ causes inactivation of eEF2K. Thus, multisite phospho-regulation of eEF2K may be important for proper control of eEF2K activity and protein translation.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

EEF2K

Molecular Weight

105

Immunogen

Phospho-eEF2K (Ser-500) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-500 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human recombinant eEF2K autophosphorylated in the presence of Ca2+ and Calmodulin. This reactivity is removed after lambda phosphatase treatment. In addition, the antibody detects eEF2K after calyculin A treatment of mouse C2C12 cells, but not in control cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-500

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

eEF-2, eEF-2K, CaMK-III, eukaryotic elongation factor

UniProt ID

O00418

Shipping

Blue Ice

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat
Additional Publications: Unspecified

Western Blot: Human
PMID	Dilution	Publication
27956550	1:1000	Tavares, C.D., et al. 2017. Signal Integration at Elongation Factor 2 Kinase: THE ROLES OF CALCIUM, CALMODULIN, AND SER-500 PHOSPHORYLATION. Journal of Biological Chemistry, 2032-2045.
22329831	not listed	Tavares, C.D., et al. 2012. Calcium/calmodulin stimulates the autophosphorylation of elongation factor 2 kinase on Thr-348 and Ser-500 to regulate its activity and calcium dependence. Biochemistry, 2232-2245.

Western Blot: Mouse
PMID	Dilution	Publication
27821870	1:500	Fuchs, T, et al. 2017. Disinhibition of somatostatin-positive GABAergic interneurons results in an anxiolytic and antidepressant-like brain state. Molecular psychiatry, 920-930.

Western Blot: Rat
PMID	Dilution	Publication
33191388	1:200	Kodama, T, et al. 2021. Age-dependent increase in activity of eukaryotic elongation factor 2 kinase in mesenteric arteries from spontaneously hypertensive rats. The Journal of veterinary medical science, 42-47.
25770246	not listed	Kameshima, S., et al. 2015. Eukaryotic elongation factor 2 kinase mediates monocrotaline-induced pulmonary arterial hypertension via reactive oxygen species-dependent vascular remodeling. American Journal of Physiology. Heart and Circulatory Physiology, H1298-H1305.

Additional Publications: Unspecified
PMID	Publication
25353634	Knight, J.R., et al. 2015. Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation. Biochemical Journal, 227-238.

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Anti-eEF2K (Ser-500), Phosphospecific Antibody

SKU: EP4451

Product Details

Product Specific References for Applications and Species

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