Anti-E-Cadherin (Cytoplasmic) Antibody (M168)

Name: Anti-E-Cadherin (Cytoplasmic) Antibody
Brand: PhosphoSolutions
SKU: CM1681
Price: 379.0 USD
Availability: InStock

Our E-Cadherin (Cytoplasmic) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat E-Cadherin (Cytoplasmic) and is Protein A purified. It is great for use in WB, ICC, IHC, IP.

Western blot image of human A431 cells treated with pervanadate (1 mM) for 30 min (lanes 1 & 3) then treated with akaline phosphatase (lanes 2 & 4). Blots were probed with anti-E-Cadherin (Cytoplasmic) and anti-N-Cadherin (Tyr-860)/E-Cadherin (Tyr-835) conserved site.

Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-E-Cadherin (CM1681) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

Human 3D differentiated epidermis was incubated with roflumilast (Rof, 100nM) or vehicle (0.1% DMSO; control) for 30 min followed by the stimulation with TGFβ1 (10 ng/ml) for 48 h (collagen type I and E cadherin proteins (Cat. CM1681)

Western blot image of human A431 cells treated with pervanadate (1 mM) for 30 min (lanes 1 & 3) then treated with akaline phosphatase (lanes 2 & 4). Blots were probed with anti-E-Cadherin (Cytoplasmic) and anti-N-Cadherin (Tyr-860)/E-Cadherin (Tyr-835) conserved site.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

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Data Sheet

SKU: CM1681

Volume: 100 µL

Variant

Price:

$379.00

Quantity:

Ships: 1-2 business days

Product Details

Target

E-Cadherin (Cytoplasmic)

Scientific Background

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M168

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

CDH1

Molecular Weight

120

Immunogen

Clone (M168) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the cytoplasmic region. This sequence is highly conserved in human and rat E-cadherin.

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:50
ICC: 1:250
IP: 1:100

Conjugate

Unconjugated

Specificity

This E-cadherin antibody detects a 120 kDa* protein in human A431 cells, and does not cross-react with VE-cadherin or N-cadherin.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Uvomorulin, Cadherin-1, CTF1, CTF2, CTF3, CD324, Epithelial Cadherin

UniProt ID

P09803

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Anti-E-Cadherin (Cytoplasmic) Antibody (M168)

SKU: CM1681

Product Details

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